FastQCFastQC Report
Thu 26 May 2016
SRR1512522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979381
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT55500.2803906877958311No Hit
GTCCTAAAGTGTGTATTTCTCATTT53650.2710443315359701No Hit
CTGTAGGACGTGGAATATGGCAAGA51060.25795943277216465No Hit
GTATCAACGCAGAGTACTTTTTTTT46940.23714484477723088No Hit
CTTTAGGACGTGAAATATGGCGAGG42730.215875569180466No Hit
GTCCTACAGTGTGCATTTCTCATTT31080.15701878516566542No Hit
TATCAACGCAGAGTACTTTTTTTTT28430.1436307613339726No Hit
GGTATCAACGCAGAGTACTTTTTTT28390.143428677955381No Hit
CTGAAGGACCTGGAATATGGCGAGA22910.11574325508833316No Hit
CCCATGTACTCTGCGTTGATACCAC21030.10624533629452844No Hit
GTACATGGGAAGCAGTGGTATCAAC20630.10422450250861255No Hit
GTCCTTCAGTGTGCATTTCTCATTT20330.10270887716917561No Hit
ATTTAGAAATGTCCACTGTAGGACG20280.10245627294593614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG400.005360399414.217485
TCCAACG2650.013.59155418
CCAACGA1800.012.11210719
TAGGACC10100.012.0121284
GTCCTAT2700.011.700271
CCAACGT1302.7084752E-911.66651219
GGCGAGG13250.011.44638919
GTGTAGG4450.011.3140961
GTCCTAG3000.011.1883831
GACGTCA600.005976259711.058327
TAGGACA7600.010.8501834
GGACCTG18000.010.847416
TGTAGGA32200.010.832642
TAGAACG1053.5633548E-610.8323654
CCACCTT8100.010.7728713
GTGGACG803.8602308E-410.6606789
GTATACG650.00800648410.6314961
AGGACCT18600.010.5994115
AGAACGT909.740118E-510.53146655
GTAGGAC32500.010.3240783
Produced by FastQC (version 0.11.5)