FastQCFastQC Report
Thu 26 May 2016
SRR1512522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979381
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63510.320857884358797No Hit
CTGTAGGACGTGGAATATGGCAAGA58580.29595110794738355No Hit
CTTTAGGACGTGAAATATGGCGAGG50230.25376620266638916No Hit
GTCCTACAGTGGACATTTCTAAATT48250.24376307542610542No Hit
TATCAACGCAGAGTACTTTTTTTTT47990.24244953346526008No Hit
GTCCTAAAGTGTGTATTTCTCATTT46890.23689224055399136No Hit
GGTATCAACGCAGAGTACTTTTTTT45910.2319411977784974No Hit
GTACATGGGAAGCAGTGGTATCAAC32130.16232347385369467No Hit
CTGAAGGACCTGGAATATGGCGAGA30470.15393701364214368No Hit
GTCCTACAGTGTGCATTTCTCATTT25330.12796929949312438No Hit
ATTTAGAAATGTCCACTGTAGGACG25280.12771669526988488No Hit
TTTCTAAATTTTCCACCTTTTTCAG24130.12190679813537666No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23180.1171073178938264No Hit
CTGTAGGACCTGGAATATGGCGAGA21770.10998387879847286No Hit
GAATATGGCAAGAAAACTGAAAATC21610.1091755452841065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG707.247496E-613.5745895
GCCTCGA1457.2759576E-1212.44582616
CTCGAAC1301.9826984E-1012.42031918
TCGAACT1251.4169927E-912.15668619
GACCGGG550.003071156612.0897517
CCAACGA1353.7289283E-1011.959702519
TAGGACC9100.011.9035684
TCCAACG2100.011.75929318
GGCGAGG14700.011.75869819
CCTCGAA1552.5465852E-1111.64286917
AGTCGTC2500.011.0189458
CCACCTT8000.010.92367813
TTTATAC2550.010.8058913
CACCTTT8400.010.74220514
CAAGTCG2300.010.7381026
TTAGTAC803.7653104E-410.6894473
TAGAAAT9500.010.5021794
AAGTCGT2450.010.4683867
AGGACGT33400.010.4126035
ATTTAGA9600.010.4035621
Produced by FastQC (version 0.11.5)