FastQCFastQC Report
Thu 26 May 2016
SRR1512521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620679
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT68520.2614589577739204No Hit
CTGTAGGACGTGGAATATGGCAAGA66310.25302602875056424No Hit
GTCCTAAAGTGTGTATTTCTCATTT63960.24405888702889594No Hit
GTATCAACGCAGAGTACTTTTTTTT61060.23299305256385844No Hit
GTACATGGGAAGCAGTGGTATCAAC55830.21303639247691153No Hit
CTTTAGGACGTGAAATATGGCGAGG54940.20964032603764138No Hit
GGTATCAACGCAGAGTACTTTTTTT37960.1448479573423529No Hit
CCCATGTACTCTGCGTTGATACCAC37720.14393216414524634No Hit
TATCAACGCAGAGTACTTTTTTTTT36360.13874266936164253No Hit
GTCCTACAGTGTGCATTTCTCATTT31520.12027417321999374No Hit
CTGAAGGACCTGGAATATGGCGAGA29370.11207019249591423No Hit
GTCCTTCAGTGTGCATTTCTCATTT26810.10230173172677767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC350.002205066816.2474927
TAGGACC11150.013.8552984
AGGATCG500.001526816413.26878455
ACCGTCC604.2635185E-412.60576258
AAGGCGT1103.9448423E-812.0625326
AGGACCT23100.011.2009225
GGCGAGG17250.011.19896719
GCCGGTT600.005967513711.06071411
GGACCTG23350.010.878056
TGTAGGA40650.010.8418612
GTATAAG2850.010.7692861
GTCCTAC35350.010.7550351
AATCCCG1355.965376E-810.52190619
GTAGGAC39400.010.5122743
GTATTAA3050.010.3877181
GCCCTAC2200.010.3508311
TCCTACA39000.010.3041622
CTGTAGG36400.010.2815941
AGGACGT40050.010.270475
GGACGTG39250.010.2383356
Produced by FastQC (version 0.11.5)