FastQCFastQC Report
Thu 26 May 2016
SRR1512521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620679
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82140.31343022170971724No Hit
CTGTAGGACGTGGAATATGGCAAGA77070.2940840904208413No Hit
GTCCTACAGTGGACATTTCTAAATT75980.2899248629839824No Hit
GTCCTAAAGTGTGTATTTCTCATTT70530.2691287257996878No Hit
CTTTAGGACGTGAAATATGGCGAGG67980.2593984230804307No Hit
GTACATGGGAAGCAGTGGTATCAAC63780.24337204213106603No Hit
TATCAACGCAGAGTACTTTTTTTTT53310.20342056390729274No Hit
GGTATCAACGCAGAGTACTTTTTTT52630.2008258165154908No Hit
CCCATGTACTCTGCGTTGATACCAC45640.17415333964976254No Hit
CTGAAGGACCTGGAATATGGCGAGA36430.13900977571079862No Hit
GTCCTACAGTGTGCATTTCTCATTT34190.13046237253780413No Hit
ATTTAGAAATGTCCACTGTAGGACG28550.10894123240580017No Hit
GTCCTTCAGTGTGCATTTCTCATTT26990.10298857662460759No Hit
GAGTACATGGGAAGCAGTGGTATCA26930.10275962832533095No Hit
TTTCTAAATTTTCCACCTTTTTCAG26630.10161488682894776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC402.7633994E-416.6239156
TAGGACC10950.013.27564054
GTCGAGG1153.8016879E-1013.21249419
TGTCGAG1405.2750693E-1112.21069118
GCGTAGA550.003038577512.107221
TCCAACG907.4904165E-611.60769418
ATAGGAC3650.010.9324633
AGGACCT23700.010.9047395
GTCCTAT3500.010.8717881
GGACCTG23800.010.6169556
GGCGAGG17400.010.26056519
TTAGGAC25200.010.2171633
GTCCTAA21500.010.1764991
TAGAAAT10750.010.1640674
TGTAGGA39900.010.14374352
AGGACGT41550.010.0389495
GGACGTG41050.09.973776
GTCCTAC35500.09.9147651
CTGTAGG39000.09.85430051
TGGCGAG40150.09.79288418
Produced by FastQC (version 0.11.5)