FastQCFastQC Report
Thu 26 May 2016
SRR1512520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654966
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81050.305276979064892No Hit
GTCCTACAGTGGACATTTCTAAATT71110.26783770488962944No Hit
GTCCTAAAGTGTGTATTTCTCATTT68740.2589110369021675No Hit
CTGTAGGACGTGGAATATGGCAAGA67180.2530352554420659No Hit
CTTTAGGACGTGAAATATGGCGAGG56590.2131477389917611No Hit
CCCATGTACTCTGCGTTGATACCAC55950.21073716198248865No Hit
GTATCAACGCAGAGTACTTTTTTTT49940.1881003372547897No Hit
GTCCTACAGTGTGCATTTCTCATTT37770.14226170881284356No Hit
GAGTACATGGGAAGCAGTGGTATCA34380.12949318371685364No Hit
CTGAAGGACCTGGAATATGGCGAGA31270.11777928606242039No Hit
GGTATCAACGCAGAGTACTTTTTTT30140.11352311103042373No Hit
TATCAACGCAGAGTACTTTTTTTTT29090.10956825812458615No Hit
GTCCTTCAGTGTGCATTTCTCATTT27550.10376780719602435No Hit
ATTTAGAAATGTCCACTGTAGGACG26650.100377933276735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA551.9991738E-413.7867826
TAGGACC12500.013.2697764
ACCGTCC854.1518233E-612.2383938
CCGCACT701.114882E-412.186534
TCCAACG2650.012.15086618
AAGCGAC550.00312216212.0634357
TGTCGAG1650.012.05339818
ACAGTCG550.00317563212.0361068
ACTGCGC658.338424E-411.6393118
GGCGAGG18250.011.47015519
ATGTCGA1950.011.18876717
AGGACCT25900.011.125245
CAGTACG600.005977866211.0581464
GGACCTG25250.010.8485586
AATCCCG4050.010.75827719
CACCTTT10250.010.55122714
GATATAC7850.010.469021
ACTGTTC8500.010.4582638
GTACCGT1002.4611261E-510.4262536
ACCTTTT10100.010.42614115
Produced by FastQC (version 0.11.5)