FastQCFastQC Report
Thu 26 May 2016
SRR1512520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654966
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC92580.3487050304975657No Hit
CTGTAGGACGTGGAATATGGCAAGA81290.30618094544336916No Hit
GTCCTACAGTGGACATTTCTAAATT78660.29627498054589024No Hit
GTCCTAAAGTGTGTATTTCTCATTT73760.27781900031864815No Hit
CTTTAGGACGTGAAATATGGCGAGG68430.2577434136633011No Hit
CCCATGTACTCTGCGTTGATACCAC68280.25717843467675294No Hit
GTATCAACGCAGAGTACTTTTTTTT64060.2412836925218628No Hit
TATCAACGCAGAGTACTTTTTTTTT42120.15864609942274213No Hit
GGTATCAACGCAGAGTACTTTTTTT42040.15834477729658308No Hit
GAGTACATGGGAAGCAGTGGTATCA41140.15495490337729373No Hit
GTCCTACAGTGTGCATTTCTCATTT40360.15201701264724296No Hit
CTGAAGGACCTGGAATATGGCGAGA38960.1467438754394595No Hit
ATTTAGAAATGTCCACTGTAGGACG31070.11702598074702275No Hit
CTGTAGGACCTGGAATATGGCGAGA30800.11600901857123594No Hit
GTCCTTCAGTGTGCATTTCTCATTT30110.11341011523311409No Hit
TTTCTAAATTTTCCACCTTTTTCAG29300.11035922870575367No Hit
GAATATGGCAAGAAAACTGAAAATC26950.10150789124983145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13000.013.668114
TAGGCCC1103.8073267E-812.093324
CTGGTCG658.0297794E-411.6917739
TACGGCG658.04375E-411.68935116
CGGCGAG658.0488354E-411.68847118
GGCGAGG18000.011.55345319
CTTAGAC1751.8189894E-1211.4020593
TAGAAAT13150.011.1276934
TTTAGAA13850.011.1139182
GGACCGT600.00588033511.082626
ATTTAGA12550.011.0679711
AATGTCC12650.011.03902158
AAATGTC12250.010.9341957
CACCTTT11800.010.86630514
GAATACG1053.4744426E-610.85562513
AGGACCT28050.010.8060765
GAAATGT13300.010.7850196
ACGGCGA803.7820538E-410.68459717
AGGACGT43250.010.5893945
ACCTTTT11950.010.57094715
Produced by FastQC (version 0.11.5)