FastQCFastQC Report
Thu 26 May 2016
SRR1512519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608368
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64700.2480478214730437No Hit
GTCCTACAGTGGACATTTCTAAATT49650.19034890782282254No Hit
CTGTAGGACGTGGAATATGGCAAGA44760.17160155315507628No Hit
GGTATCAACGCAGAGTACTTTTTTT39050.14971047030173656No Hit
GTCCTAAAGTGTGTATTTCTCATTT37440.14353802837636406No Hit
TATCAACGCAGAGTACTTTTTTTTT36300.13916747943541707No Hit
GTCCTACAGTGTGCATTTCTCATTT31740.12168528367162916No Hit
CTTTAGGACGTGAAATATGGCGAGG31290.11996006698441325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC250.0061167418.954786
TCCAACG2350.014.50793518
TACCGCC400.00536448914.2160867
CCAACGA1600.013.615355519
TAGGACC10200.013.3798464
CGGTTTC1457.2759576E-1212.42774913
CCGGTTT1457.2759576E-1212.42319812
GCCGGTT1603.6379788E-1211.84947811
GACTGCG658.188629E-411.6644817
CAGATCG658.1978965E-411.6629099
GGTATCA15100.011.4749721
CTAATAC2400.011.0569563
GTCCTAC25250.011.0188291
AGGACGT27700.011.0170395
GGCGAGG11350.011.01537219
GTAGGAC29800.010.9403433
AGGACCT19100.010.8667475
ACTGCGT700.001551777910.8061128
TTAGGAC16700.010.782663
GGACGTG27900.010.700286
Produced by FastQC (version 0.11.5)