FastQCFastQC Report
Thu 26 May 2016
SRR1512519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608368
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83380.31966348306680653No Hit
CTGTAGGACGTGGAATATGGCAAGA54640.20947964397661678No Hit
GGTATCAACGCAGAGTACTTTTTTT54540.20909626249056878No Hit
GTCCTACAGTGGACATTTCTAAATT53830.20637425393962816No Hit
TATCAACGCAGAGTACTTTTTTTTT52950.20300049686240593No Hit
GTCCTAAAGTGTGTATTTCTCATTT40760.15626629371315703No Hit
CTTTAGGACGTGAAATATGGCGAGG38760.14859866399219743No Hit
GTCCTACAGTGTGCATTTCTCATTT34410.13192156934911026No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27620.10588996644645234No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAA250.005985678619.0260051
CGGAATA500.001500182813.29981111
TAGGACC10500.012.8503944
AGGACGT26000.011.5125455
GGACGTG25400.011.4442826
ACCGTGC752.075115E-411.3998398
TGTAGGA32400.011.3199122
GTAGGAC30200.011.295273
TCCAACG2450.011.24021218
GGTATCA17300.011.2176441
CGAAATC1451.2205419E-911.13691913
GTCCTAC30500.011.0725111
CCAACGA1553.1650416E-1011.02681519
AGTGCGG700.001492165510.85698910
GTAGAAC9050.010.8142833
AGGACCT19250.010.8105425
TAGGACG28150.010.7678674
GACGTGG15100.010.6952127
TATGTCG1252.2512359E-710.63678916
CTGTAGG32750.010.6313251
Produced by FastQC (version 0.11.5)