FastQCFastQC Report
Thu 26 May 2016
SRR1512518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62540.22018878353970897No Hit
GTCCTACAGTGGACATTTCTAAATT54560.19209306091983563No Hit
CTGTAGGACGTGGAATATGGCAAGA50520.1778691612476191No Hit
GTCCTAAAGTGTGTATTTCTCATTT48430.17051075770431892No Hit
CTTTAGGACGTGAAATATGGCGAGG40700.14329522689584515No Hit
TATCAACGCAGAGTACTTTTTTTTT38670.13614806938728088No Hit
GGTATCAACGCAGAGTACTTTTTTT37730.1328385481764186No Hit
GTCCTACAGTGTGCATTTCTCATTT29340.10329931098585005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10650.013.0826774
TACACCG957.6197466E-812.970265
CGTGCGC751.5137452E-512.63858210
AAGCCGT604.173725E-412.6376886
CGGTTTC2200.012.50270513
CCGGTTT2150.012.34684612
TCCAACG2850.011.96386818
GGCGAGG11650.011.14000619
TGTAGGA33950.011.083572
AGGACGT30500.011.0631565
CGATACA600.005978724511.0579774
GTAGGAC33250.010.97483353
GCCGGTT2100.010.83307111
TGCCGGT2100.010.83307110
GGACGTG30850.010.75336
TAGGACT5500.010.684594
TAAGACT3200.010.663054
GACGTGG16700.010.6133877
GTGTAGG4300.010.5817251
CTGTAGG31650.010.4385411
Produced by FastQC (version 0.11.5)