FastQCFastQC Report
Thu 26 May 2016
SRR1512518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840290
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT81600.2872946072408099No Hit
CTGTAGGACGTGGAATATGGCAAGA60430.21275996465149688No Hit
GTCCTACAGTGGACATTTCTAAATT59750.2103658429244901No Hit
TATCAACGCAGAGTACTTTTTTTTT53060.1868119100514384No Hit
GGTATCAACGCAGAGTACTTTTTTT51510.18135472082076126No Hit
GTCCTAAAGTGTGTATTTCTCATTT51360.18082660573392154No Hit
CTTTAGGACGTGAAATATGGCGAGG47920.16871516640906387No Hit
GTACATGGGAAGCAGTGGTATCAAC31540.11104499892616598No Hit
GTCCTACAGTGTGCATTTCTCATTT31320.11027043013213439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11350.013.0602954
AATCCCG2750.011.05048919
ACACCGT1053.4702516E-610.8567916
GTATAGG2650.010.7704911
TGTAGGA33850.010.7508162
AGGACGT30150.010.7476685
GGACGTG29900.010.707016
TATACCG803.762835E-410.6905425
GACGTGG15550.010.6296937
AGGACCT20750.010.624715
GGACCTG19600.010.5175166
GTAGGAC33600.010.37834553
CACCTTT9350.010.36111114
TTAGGAC17600.010.3115043
GGCGAGG11800.010.30130319
CTGTAGG34600.010.1463621
GTCCTAC29550.010.1417571
CAACCGT750.002649437410.1330056
CGTGGAA16350.010.1094079
CGGTTTC1606.193659E-910.09235713
Produced by FastQC (version 0.11.5)