FastQCFastQC Report
Thu 26 May 2016
SRR1512517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195722
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55960.17510909897669447No Hit
GTCCTACAGTGGACATTTCTAAATT49100.1536429013537473No Hit
CTGTAGGACGTGGAATATGGCAAGA41540.12998627540192795No Hit
GTCCTAAAGTGTGTATTTCTCATTT39760.12441632907993873No Hit
TATCAACGCAGAGTACTTTTTTTTT33760.10564122911817736No Hit
GTACATGGGAAGCAGTGGTATCAAC33630.10523443528567254No Hit
CTTTAGGACGTGAAATATGGCGAGG33500.10482764145316771No Hit
GGTATCAACGCAGAGTACTTTTTTT33400.10451472312047168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT2550.013.7450619
TCCAACG5000.013.63932918
TAGGACC11300.012.9165294
CCAACGA2900.012.412827519
CGATCCA550.003120816812.06425210
GTCCTAG3950.011.5243831
GTCCTAT3450.011.473551
TAAGACG1001.9868166E-611.3732574
TGTAGGA28700.011.1290582
GGACGTG24850.011.060516
CTGTAGG26900.010.9627861
AGGACGT25850.010.9259525
GTAGGAC28200.010.85567953
GACGTGG13050.010.8212987
AGGACCT19900.010.5731285
GTCCTAC28200.010.5275931
GTGTAGG5750.010.4982981
GACGTGA12750.010.48123557
GGCGAGG10150.010.45290819
TATGTCG1002.4316962E-510.43779416
Produced by FastQC (version 0.11.5)