FastQCFastQC Report
Thu 26 May 2016
SRR1512517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3195722
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77230.2416668283411386No Hit
GTCCTACAGTGGACATTTCTAAATT52600.1645950429981081No Hit
CTGTAGGACGTGGAATATGGCAAGA51040.15971351700805014No Hit
TATCAACGCAGAGTACTTTTTTTTT49980.15639658268147227No Hit
GGTATCAACGCAGAGTACTTTTTTT48180.15076405269294388No Hit
GTCCTAAAGTGTGTATTTCTCATTT43940.13749631538663248No Hit
CTTTAGGACGTGAAATATGGCGAGG42490.13295899956254018No Hit
GTACATGGGAAGCAGTGGTATCAAC37890.11856475625852311No Hit
GTCCTACAGTGTGCATTTCTCATTT33720.10551606178509897No Hit
TTGTAGAACAGTGTATATCAATGAG33520.1048902251197069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11800.012.7245524
CCAACGA2250.012.66134619
TCCAACG4400.012.51864318
TGAGTCG701.0882255E-412.2183325
TGTAGGA32700.011.246142
GTAGGAC31400.011.2275383
AGGACCT21150.011.0982385
GACGTGG14600.011.0606337
GGACCTG20750.010.75838856
AGGACGT27150.010.7457325
GGACGTG26400.010.7228396
AAGGCGT1951.8189894E-1210.7173056
ACTGTTC13950.010.6907798
GATATAC13000.010.6091341
GTCCTAC27600.010.4765841
CTGTAGG33950.010.4501921
GCGTGCG1002.4029727E-510.4490449
TGGCGAG27700.010.14831418
TGTAGAA16400.010.1399182
TAGGCCG750.002641610810.136695
Produced by FastQC (version 0.11.5)