FastQCFastQC Report
Thu 26 May 2016
SRR1512516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161111
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38940.3353684531453065No Hit
GGTATCAACGCAGAGTACTTTTTTT27080.23322490270094764No Hit
TATCAACGCAGAGTACTTTTTTTTT24130.20781820170509108No Hit
GTCCTAAAGTGTGTATTTCTCATTT19940.17173207384995923No Hit
GTCCTACAGTGGACATTTCTAAATT18900.16277513519379283No Hit
CTGTAGGACGTGGAATATGGCAAGA18730.16131102022115026No Hit
CTTTAGGACGTGAAATATGGCGAGG16870.1452918799322373No Hit
GTACATGGGAAGCAGTGGTATCAAC14810.12755025144021545No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12710.10946412530757181No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12220.10524402920995493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCT350.002204569816.2469397
GCGCAGA755.996717E-714.5350691
TAGGACC4350.013.7258624
GGATAAT751.3987886E-411.8923291
GTATAGG1102.870529E-711.7121431
GTATTAC853.4556702E-511.6591461
TAAGCCT1501.8371793E-1011.3728564
CTAGGAC1803.6379788E-1211.0569443
GCCTTAT1103.643041E-610.8112091
GTCCTAG1654.4383341E-1010.8112091
AGGACCT8350.010.5556465
GGATAGA854.4643975E-410.4932321
GTTACAA2300.010.31041915
ATTTAGA3950.010.2866151
GGTATCA10450.010.2421981
TGGCGAG13350.010.21572418
TGAGTTA3350.010.18817212
TAATCCC2350.010.082325
AAATGTC3300.010.0517677
AACACTA856.7362183E-410.0348745
Produced by FastQC (version 0.11.5)