FastQCFastQC Report
Thu 26 May 2016
SRR1512516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161111
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50900.4383732476912199No Hit
GGTATCAACGCAGAGTACTTTTTTT36150.3113397427119371No Hit
TATCAACGCAGAGTACTTTTTTTTT34870.30031581821204No Hit
CTGTAGGACGTGGAATATGGCAAGA21970.18921532911151476No Hit
GTCCTAAAGTGTGTATTTCTCATTT21410.18439236214280977No Hit
CTTTAGGACGTGAAATATGGCGAGG21350.1838756156818771No Hit
GTCCTACAGTGGACATTTCTAAATT20190.17388518410384537No Hit
GTACATGGGAAGCAGTGGTATCAAC17430.1501148469009423No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16970.14615312403379177No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15620.13452632866280656No Hit
GTCCTACAGTGTGCATTTCTCATTT12370.10653589536228664No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12160.10472728274902228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGA400.005282008614.2483859
GCCGGTT655.45597E-513.15235511
CCAACGA604.1019998E-412.66250419
GAGCCGT550.003070247112.0895396
TAGGACC4600.011.9815474
GGCGAGG5750.011.56141719
GTTAGAC752.0685044E-411.4026373
TAATACC1354.7002686E-911.2623494
GTATTAG600.00580224311.1016841
GTCTTAG1201.2449527E-711.1016841
TAGGGTG600.005862154511.0863745
CTAGGAC1803.6379788E-1211.0858973
GGTATCA12750.010.9710751
GTCCTAA6700.010.9359871
GAGTAAT1053.395162E-610.8751181
CCGGTTT700.001492319910.85591312
CCTAGAC1158.781135E-710.7416153
GTATAAC803.7119348E-410.7051941
AATCCCG803.780591E-410.68398819
TTAGGAC8350.010.3556893
Produced by FastQC (version 0.11.5)