FastQCFastQC Report
Thu 26 May 2016
SRR1512515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121629
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58440.18720994711415098No Hit
GTACATGGGAAGCAGTGGTATCAAC49020.15703339506392336No Hit
CCCATGTACTCTGCGTTGATACCAC37010.11855989292769897No Hit
GGTATCAACGCAGAGTACTTTTTTT36230.11606119753500496No Hit
GTCCTACAGTGGACATTTCTAAATT35740.11449150427549205No Hit
TATCAACGCAGAGTACTTTTTTTTT34370.11010277006011926No Hit
GTCCTAAAGTGTGTATTTCTCATTT32960.10558589761948008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.011.4644284
ATACCGT1001.9879117E-611.3727126
TATACCG855.4582022E-511.1497175
GTCCTAA10400.010.7693471
GTCCTAT3350.010.6512841
TTAGGAC13600.010.3831743
GGACGTG19700.010.2950946
AGGACGT19900.010.1916265
GTCCTAC17650.010.1643161
CTTACGC750.002702023110.1090773
GACGTGG10150.09.9908077
TAGGACG21150.09.8581424
TGTAGGA20000.09.6671162
CTTATAC3250.09.6230643
GTAGGAC19000.09.5770213
GGCGAGG9750.09.42304719
TATACTG4250.09.3657635
GTATAGG3500.09.3452341
GTATAAG4050.09.2997831
GTATTAA3950.09.2842941
Produced by FastQC (version 0.11.5)