FastQCFastQC Report
Thu 26 May 2016
SRR1512515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121629
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71050.22760552262937075No Hit
GTACATGGGAAGCAGTGGTATCAAC58480.18733808533941732No Hit
TATCAACGCAGAGTACTTTTTTTTT47700.1528048336301335No Hit
GGTATCAACGCAGAGTACTTTTTTT45190.14476415999466946No Hit
CCCATGTACTCTGCGTTGATACCAC43530.13944642364611554No Hit
CTGTAGGACGTGGAATATGGCAAGA39700.12717718857686164No Hit
GTCCTACAGTGGACATTTCTAAATT35940.11513219540182386No Hit
GTCCTAAAGTGTGTATTTCTCATTT32310.10350365145890175No Hit
CTTTAGGACGTGAAATATGGCGAGG32280.10340754778995198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGT500.001498337713.302124
TAGGACC7050.012.2643664
TACGACT550.003065975812.0928364
TTAGGAC13300.011.2153553
CGTCCAA600.00587934911.08296910
TATAACG803.769648E-410.6885192
AGGACGT20450.010.4076935
GTCCTAT3050.010.2913781
GGCGAGG10250.010.28400519
TGTAGGA21500.010.119572
TTATACT3850.010.1184964
TATGTCG951.6485044E-49.99790916
GGACGTG20950.09.9754866
GACGTGA11150.09.9682837
ACGTGAA12200.09.9668858
CGTGAAA11850.09.86043559
TGGCGAG20250.09.80210318
GTCTAAG3300.09.7999631
TAGGACG22350.09.7778444
GGACCGT1951.6370905E-109.7429576
Produced by FastQC (version 0.11.5)