FastQCFastQC Report
Thu 26 May 2016
SRR1512514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171767
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71820.22643529616141414No Hit
CTGTAGGACGTGGAATATGGCAAGA65200.2055636495366778No Hit
GTATCAACGCAGAGTACTTTTTTTT63310.1996048259534827No Hit
GTCCTAAAGTGTGTATTTCTCATTT61340.19339377703343277No Hit
CTTTAGGACGTGAAATATGGCGAGG54810.17280588391265816No Hit
GTACATGGGAAGCAGTGGTATCAAC53250.16788748984398916No Hit
GGTATCAACGCAGAGTACTTTTTTT38470.12128885886006127No Hit
CCCATGTACTCTGCGTTGATACCAC38390.12103663352320645No Hit
TATCAACGCAGAGTACTTTTTTTTT37310.11763159147566642No Hit
GTCCTACAGTGTGCATTTCTCATTT35350.11145207072272333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13100.012.6610054
ACCGCCC854.1618496E-612.2359418
GGACCGT701.1158324E-412.1855726
GGCGAGG18850.011.95986519
AGGACCT24550.011.6974875
TCCAACG2800.011.50039618
TAAGACG1105.1274037E-711.2008794
GGACCTG23950.011.199086
TGGCGAG39750.010.79326818
GTCCTAC35900.010.6206141
AGGACGT42200.010.6006145
TTAGGAC25300.010.4141953
GACGTGA22200.010.3742037
GTATTAA4500.010.3435281
GGACGTG41800.010.33927256
TGTAGGA42750.010.3313752
TCCTACA41100.010.307992
AGCACCG1201.5718415E-610.2674725
TAGAAAT10900.010.2602264
CCAACGA1951.6370905E-1110.20106219
Produced by FastQC (version 0.11.5)