FastQCFastQC Report
Thu 26 May 2016
SRR1512514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171767
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT84010.26486813186466723No Hit
CTGTAGGACGTGGAATATGGCAAGA78650.24796903429539432No Hit
GTCCTACAGTGGACATTTCTAAATT76090.2398978235160401No Hit
CTTTAGGACGTGAAATATGGCGAGG66330.20912633241975215No Hit
GTCCTAAAGTGTGTATTTCTCATTT65100.2052483678656093No Hit
GTACATGGGAAGCAGTGGTATCAAC62790.19796536126392641No Hit
TATCAACGCAGAGTACTTTTTTTTT57160.18021500318276848No Hit
GGTATCAACGCAGAGTACTTTTTTT54430.17160781356259774No Hit
CCCATGTACTCTGCGTTGATACCAC45710.14411525184542245No Hit
GTCCTACAGTGTGCATTTCTCATTT38030.11990161950735977No Hit
CTGAAGGACCTGGAATATGGCGAGA36030.1135959860859893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAG402.7340115E-416.6474131
TAGGACC11950.012.4836064
GGCGAGG17050.011.25058519
TACGTCT600.00586941611.0854954
GTCTAGA3100.010.7402661
AATGTCC10450.010.4542768
AGGACGT40650.010.4254875
CCACCTT10400.010.411880513
AGGACCT26150.010.3924125
CTAGGAC4400.010.3653323
GTCCTAC36350.010.3109931
TGTCCAC11800.010.30481910
TGTAGGA42550.010.294282
GGACCTG25850.010.2532836
GGACGTG39700.010.2417126
TAGAAAT11750.010.1892214
TTTAGAA12800.010.1696312
TGGCGAG39450.010.15908518
ACCTTTT10200.010.14945915
TATACCG750.00264389610.1356315
Produced by FastQC (version 0.11.5)