##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3171767 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.486261128260683 33.0 31.0 34.0 30.0 34.0 2 31.767974759810542 33.0 31.0 34.0 30.0 34.0 3 31.93423634207683 34.0 31.0 34.0 30.0 34.0 4 35.40697630059207 37.0 35.0 37.0 33.0 37.0 5 34.869173240026775 37.0 35.0 37.0 32.0 37.0 6 35.097339747844025 37.0 35.0 37.0 32.0 37.0 7 35.05515632138174 37.0 35.0 37.0 32.0 37.0 8 35.03179836349896 37.0 35.0 37.0 32.0 37.0 9 36.61749239461789 39.0 37.0 39.0 32.0 39.0 10 36.560964597967 39.0 37.0 39.0 32.0 39.0 11 36.59772801722195 39.0 37.0 39.0 32.0 39.0 12 36.52364722881599 39.0 37.0 39.0 32.0 39.0 13 36.4848398384875 39.0 37.0 39.0 32.0 39.0 14 37.76567950924516 40.0 38.0 41.0 32.0 41.0 15 37.76379790823223 40.0 38.0 41.0 32.0 41.0 16 37.74531105216745 40.0 38.0 41.0 32.0 41.0 17 37.72415155337703 40.0 38.0 41.0 32.0 41.0 18 37.669873922012556 40.0 38.0 41.0 32.0 41.0 19 37.698110233191784 40.0 38.0 41.0 32.0 41.0 20 37.67394546951274 40.0 38.0 41.0 32.0 41.0 21 37.62040811951193 40.0 38.0 41.0 32.0 41.0 22 37.55781651048138 40.0 38.0 41.0 32.0 41.0 23 37.450962192367854 40.0 38.0 41.0 32.0 41.0 24 37.41611631623634 40.0 38.0 41.0 32.0 41.0 25 37.360164223916826 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18530543136600386 1101 2 0.395261178237039 1101 3 0.32347396702950704 1101 4 0.27056362489819463 1101 5 -0.024175415080648577 1101 6 0.2932730299642756 1101 7 0.3808060314985724 1101 8 0.41746341912439533 1101 9 0.5254652200597718 1101 10 0.4907586017125851 1101 11 0.38911394970764945 1101 12 0.43328923000379405 1101 13 0.5651177508730427 1101 14 0.6343254723942948 1101 15 0.5772661428751533 1101 16 0.647079311106431 1101 17 0.6267694540812769 1101 18 0.5256527012043648 1101 19 0.49983894760723047 1101 20 0.546278255043994 1101 21 0.624803604417302 1101 22 0.5753801151052258 1101 23 0.5601985540978376 1101 24 0.5921443769122234 1101 25 0.5340555538101768 1102 1 0.14951098801923735 1102 2 0.2925123785384507 1102 3 0.30920050766314233 1102 4 0.3516881423952327 1102 5 0.05834247859963426 1102 6 0.2058340752576484 1102 7 0.3594749028417894 1102 8 0.39761737486676196 1102 9 0.4516111312347064 1102 10 0.42361190646460045 1102 11 0.40348463634003195 1102 12 0.46923756286432905 1102 13 0.5125699549792913 1102 14 0.6731076535155367 1102 15 0.660439791692113 1102 16 0.7114970200457122 1102 17 0.7489034573537054 1102 18 0.6540659117498748 1102 19 0.6533792894719141 1102 20 0.6686625472386964 1102 21 0.7250058224131593 1102 22 0.6774807023689533 1102 23 0.8203261950093008 1102 24 0.7856134825575083 1102 25 0.662103679191226 1103 1 -0.015139210186593033 1103 2 0.10913943428914408 1103 3 0.11030687911591741 1103 4 0.1951859020427804 1103 5 -0.14727962453581256 1103 6 0.1494308418341106 1103 7 0.3093853027055218 1103 8 0.40905247445485315 1103 9 0.4581546986465028 1103 10 0.41799016982756854 1103 11 0.4212643470575941 1103 12 0.44941322729977173 1103 13 0.4834873458875464 1103 14 0.609517473439162 1103 15 0.6710244404347918 1103 16 0.6118725413024464 1103 17 0.531361691368879 1103 18 0.6201691714565882 1103 19 0.6640753711850067 1103 20 0.5730679803508139 1103 21 0.56869738991292 1103 22 0.5575108494814174 1103 23 0.6490267556820655 1103 24 0.6358665060821735 1103 25 0.6031325135412686 1104 1 -0.1706347206996064 1104 2 -0.04135948808842471 1104 3 0.03553263573024168 1104 4 0.03931108368254854 1104 5 -0.32185263895713234 1104 6 -0.1294806815348224 1104 7 8.034669941636707E-4 1104 8 0.055783270543876995 1104 9 0.06984119248002685 1104 10 0.19630006832416314 1104 11 0.09286345973021781 1104 12 0.14771906066978602 1104 13 0.12267955013225418 1104 14 0.06705691435082883 1104 15 0.09162070058764726 1104 16 0.19560643804684474 1104 17 0.3128036087501229 1104 18 0.2727743007244925 1104 19 0.2698295699963822 1104 20 0.307441155505785 1104 21 0.30645271226567417 1104 22 0.301523191801806 1104 23 0.3420364014338375 1104 24 0.379607146847313 1104 25 0.23955026022739645 1105 1 0.10828351985845686 1105 2 0.2127350067088507 1105 3 0.1653295927599956 1105 4 0.23914914677097698 1105 5 -0.07792425125128943 1105 6 0.079914790232543 1105 7 0.24499754603451152 1105 8 0.30805184404231767 1105 9 0.34231152615094373 1105 10 0.2584356613528769 1105 11 0.28940425422123184 1105 12 0.25293935183443494 1105 13 0.24718694491783566 1105 14 0.40525450086467174 1105 15 0.4602519031866379 1105 16 0.43272373490059834 1105 17 0.4238538026700098 1105 18 0.42963646432164637 1105 19 0.37249035347707604 1105 20 0.28339248050542665 1105 21 0.33941139617603966 1105 22 0.35345528092277334 1105 23 0.5107492682433374 1105 24 0.5308345668887924 1105 25 0.4714119998490176 1106 1 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14 -2.1587366226026816 2314 15 -1.4931713330773917 2314 16 -1.1358213202638154 2314 17 -0.9604917703726343 2314 18 -0.8690994266357706 2314 19 -0.7750671109640734 2314 20 -0.8441142263959378 2314 21 -0.7752479222112143 2314 22 -0.8011297604938008 2314 23 -0.6191954264829818 2314 24 -0.6363317602405729 2314 25 -0.6334859498071665 2315 1 -1.0554495916576663 2315 2 -0.9524324776833559 2315 3 -0.7507559496345557 2315 4 -0.6512259625126973 2315 5 -0.43296275930512707 2315 6 -0.6514348870917956 2315 7 -0.7551676146120201 2315 8 -1.1615019028613887 2315 9 -1.1252037115718068 2315 10 -1.1443341984071012 2315 11 -1.0039791382671126 2315 12 -0.9435826564979806 2315 13 -1.0364591078516696 2315 14 -1.0261840310841066 2315 15 -1.242268374640922 2315 16 -1.2153910630199434 2315 17 -1.2272416264452275 2315 18 -1.2426078682373571 2315 19 -1.2230020683317946 2315 20 -1.0647516601050455 2315 21 -0.958839125774432 2315 22 -0.9237757309566987 2315 23 -0.8437376272963135 2315 24 -0.9975629578054637 2315 25 -0.9653287298709969 2316 1 -0.7164587876537247 2316 2 -0.7231481829520661 2316 3 -0.7879796358324604 2316 4 -0.7595184036830105 2316 5 -0.4848315346000476 2316 6 -0.7795782034511589 2316 7 -1.25714819067732 2316 8 -1.0313080619362012 2316 9 -1.0545198980262924 2316 10 -1.079320437275328 2316 11 -1.0869991712180962 2316 12 -1.1435691640762968 2316 13 -1.0643167048070268 2316 14 -1.3304574920358618 2316 15 -1.2599922162832655 2316 16 -1.326359760781365 2316 17 -1.385030105894245 2316 18 -1.3977700585894226 2316 19 -1.3925308119530726 2316 20 -1.49001206174664 2316 21 -1.7200115189615772 2316 22 -1.6335746501370352 2316 23 -1.543881436620353 2316 24 -1.548478007014161 2316 25 -1.664780902800942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31397.0 3 4.0 4 717.0 5 5264.0 6 6022.0 7 4937.0 8 3978.0 9 3521.0 10 3297.0 11 3078.0 12 2879.0 13 2812.0 14 2772.0 15 2693.0 16 2880.0 17 3111.0 18 3282.0 19 3816.0 20 4737.0 21 6326.0 22 8032.0 23 10279.0 24 12415.0 25 14546.0 26 17189.0 27 19546.0 28 23029.0 29 27540.0 30 35800.0 31 47017.0 32 61364.0 33 81256.0 34 110097.0 35 151631.0 36 227393.0 37 422723.0 38 1804387.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.745773439633304 19.715761743689953 13.539198772636226 24.999266044040514 2 14.510721240188065 20.626406209011844 41.477900447969006 23.384972102831085 3 18.522482961100202 25.110311052733962 30.23341014776558 26.13379583840026 4 12.287955767798598 17.436828932541225 38.617762623966385 31.657452675693794 5 12.753146320915429 38.3401846236921 36.17118584780894 12.735483207583531 6 31.45966074584447 37.688854717630896 18.156196427828075 12.695288108696555 7 27.67392520470414 31.692862111743263 22.67865848543846 17.95455419811413 8 24.66863449505841 37.86719018763951 20.67925711062208 16.78491820668 9 26.34120515086853 15.04299064949038 20.641723775735343 37.97408042390575 10 14.773102830617086 29.22429234572885 35.05622760986932 20.946377213784753 11 33.69725608823458 22.31276752958377 25.372140687644656 18.617835694536996 12 23.310145121329708 24.920684078535064 32.22333092810035 19.54583987203488 13 28.876355559564203 22.147368447609733 25.992904904975617 22.983371087850447 14 20.302872833266694 22.798234450539262 28.000380908656336 28.8985118075377 15 22.631162698002257 31.85102309048757 23.387254444408004 22.130559767102174 16 21.33194040995589 28.332767839236546 28.89689550047489 21.438396250332676 17 21.285046345871756 29.21505266490339 28.36882585833513 21.131075130889723 18 21.1439412595077 27.462353094378734 30.868302754304022 20.525402891809545 19 23.23254711909744 27.014276584427833 28.22050782278383 21.532668473690897 20 23.31120353143589 27.641159855107965 28.645024924796825 20.40261168865932 21 22.30040901723308 26.947876392488812 27.822346598044174 22.92936799223394 22 22.721702076676056 28.64889150932242 27.631473158335318 20.997933255666208 23 21.508174950917507 28.374143716573535 28.435687979415462 21.6819933530935 24 22.712057213427613 27.2375439945039 29.04338773207958 21.00701105998891 25 22.174852857904938 27.63616712971079 28.595097239264966 21.59388277311931 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 245.0 2 529.0 3 813.0 4 813.0 5 813.0 6 2257.0 7 3701.0 8 3701.0 9 3701.0 10 5368.0 11 7035.0 12 7035.0 13 7035.0 14 11345.5 15 15656.0 16 15656.0 17 15656.0 18 28069.5 19 40483.0 20 40483.0 21 40483.0 22 64141.0 23 87799.0 24 87799.0 25 87799.0 26 126305.0 27 164811.0 28 164811.0 29 164811.0 30 212042.0 31 259273.0 32 259273.0 33 259273.0 34 291413.0 35 323553.0 36 323553.0 37 323553.0 38 344157.0 39 364761.0 40 364761.0 41 364761.0 42 378889.5 43 393018.0 44 393018.0 45 393018.0 46 409006.0 47 424994.0 48 424994.0 49 424994.0 50 400998.5 51 377003.0 52 377003.0 53 377003.0 54 328272.5 55 279542.0 56 279542.0 57 279542.0 58 236789.0 59 194036.0 60 194036.0 61 194036.0 62 157655.5 63 121275.0 64 121275.0 65 121275.0 66 93779.5 67 66284.0 68 66284.0 69 66284.0 70 47889.0 71 29494.0 72 29494.0 73 29494.0 74 20593.5 75 11693.0 76 11693.0 77 11693.0 78 8010.0 79 4327.0 80 4327.0 81 4327.0 82 2848.0 83 1369.0 84 1369.0 85 1369.0 86 914.0 87 459.0 88 459.0 89 459.0 90 287.0 91 115.0 92 115.0 93 115.0 94 70.5 95 26.0 96 26.0 97 26.0 98 14.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1261757247616234 2 0.0037518518857154385 3 0.0017025210237700309 4 0.0044454715620661925 5 0.0415541242468315 6 0.025569343523657317 7 0.019074541099645716 8 0.013241830184878019 9 0.015637970884998804 10 0.022920977486681714 11 0.026988111043465678 12 0.02846993489748774 13 0.026609773038183446 14 0.012705851344061528 15 0.018633146760149783 16 0.01573255538631936 17 0.012453626007206708 18 0.008134267113567926 19 0.00220697169747967 20 8.197323447781632E-4 21 5.675070079233437E-4 22 0.003531154715967472 23 0.00208085902905226 24 0.002017802694838555 25 9.458450132055728E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3171767.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.80191913123996 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18948677663971 51.31630718144202 2 13.566177124535717 17.582286258884817 3 3.8821014204699016 7.5470286692568695 4 1.424017359407323 3.6911623106318086 5 0.6299707055759659 2.0411655358891956 6 0.3577912900764814 1.39113373472389 7 0.2070708392362313 0.9393011445036972 8 0.14062368708839915 0.7290147838911386 9 0.10186099101272676 0.594070893201121 >10 0.4511507358074136 5.100470948373245 >50 0.027015385119383735 1.192855358930825 >100 0.0174887335459179 2.374323975443169 >500 0.0024767826490419255 1.0423390988936438 >1k 0.0023796539114597968 2.744774885964816 >5k 3.8851492431996677E-4 1.7137652199697306 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8401 0.26486813186466723 No Hit CTGTAGGACGTGGAATATGGCAAGA 7865 0.24796903429539432 No Hit GTCCTACAGTGGACATTTCTAAATT 7609 0.2398978235160401 No Hit CTTTAGGACGTGAAATATGGCGAGG 6633 0.20912633241975215 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6510 0.2052483678656093 No Hit GTACATGGGAAGCAGTGGTATCAAC 6279 0.19796536126392641 No Hit TATCAACGCAGAGTACTTTTTTTTT 5716 0.18021500318276848 No Hit GGTATCAACGCAGAGTACTTTTTTT 5443 0.17160781356259774 No Hit CCCATGTACTCTGCGTTGATACCAC 4571 0.14411525184542245 No Hit GTCCTACAGTGTGCATTTCTCATTT 3803 0.11990161950735977 No Hit CTGAAGGACCTGGAATATGGCGAGA 3603 0.1135959860859893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.1528167106852424E-5 7 0.0 0.0 0.0 0.0 3.1528167106852424E-5 8 0.0 0.0 0.0 0.0 3.1528167106852424E-5 9 0.0 0.0 0.0 0.0 3.1528167106852424E-5 10 0.0 0.0 0.0 0.0 3.1528167106852424E-5 11 0.0 0.0 0.0 9.458450132055728E-5 3.1528167106852424E-5 12 0.0 0.0 0.0 9.458450132055728E-5 3.1528167106852424E-5 13 0.0 0.0 0.0 1.5764083553426213E-4 3.1528167106852424E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTAG 40 2.7340115E-4 16.647413 1 TAGGACC 1195 0.0 12.483606 4 GGCGAGG 1705 0.0 11.250585 19 TACGTCT 60 0.005869416 11.085495 4 GTCTAGA 310 0.0 10.740266 1 AATGTCC 1045 0.0 10.454276 8 AGGACGT 4065 0.0 10.425487 5 CCACCTT 1040 0.0 10.4118805 13 AGGACCT 2615 0.0 10.392412 5 CTAGGAC 440 0.0 10.365332 3 GTCCTAC 3635 0.0 10.310993 1 TGTCCAC 1180 0.0 10.304819 10 TGTAGGA 4255 0.0 10.29428 2 GGACCTG 2585 0.0 10.253283 6 GGACGTG 3970 0.0 10.241712 6 TAGAAAT 1175 0.0 10.189221 4 TTTAGAA 1280 0.0 10.169631 2 TGGCGAG 3945 0.0 10.159085 18 ACCTTTT 1020 0.0 10.149459 15 TATACCG 75 0.002643896 10.135631 5 >>END_MODULE