FastQCFastQC Report
Thu 26 May 2016
SRR1512513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250559
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56890.25278164224976996No Hit
GTACATGGGAAGCAGTGGTATCAAC45580.202527460955256No Hit
TATCAACGCAGAGTACTTTTTTTTT35020.15560578505162495No Hit
GGTATCAACGCAGAGTACTTTTTTT34940.1552503178099308No Hit
CCCATGTACTCTGCGTTGATACCAC32240.1432532984027524No Hit
GTCCTACAGTGGACATTTCTAAATT29480.13098967856430335No Hit
CTGTAGGACGTGGAATATGGCAAGA26680.11854832510500724No Hit
GTCCTAAAGTGTGTATTTCTCATTT25850.11486035247243018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6200.012.5337934
ATAGGAC2750.011.0275143
GTCTAAT2001.8189894E-1210.8925711
GTCCTAC15050.010.7905861
TACGACT803.858365E-410.6613664
GTCCTAA9450.010.583451
TCTAGAC2250.010.5297453
GGACCGT1106.227152E-610.3382956
TCCTACA17800.010.2221352
GCGCAGA1655.178663E-910.2024081
TATGTCG951.6660434E-49.98714216
TCGAACT1054.2380438E-59.92383519
GGTTAGG1406.009486E-79.9023381
GTAGGAC17050.09.8936373
TGTAGGA18450.09.6565482
GGACGTG16400.09.6501266
TCTGTAC3450.09.6141163
AGGACGT17050.09.5601435
CCTACAG18350.09.5542373
GTATAAG2800.09.5486831
Produced by FastQC (version 0.11.5)