FastQCFastQC Report
Thu 26 May 2016
SRR1512513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250559
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72650.3228086888635223No Hit
GTACATGGGAAGCAGTGGTATCAAC52300.23238670925756666No Hit
TATCAACGCAGAGTACTTTTTTTTT48970.21759038532204664No Hit
GGTATCAACGCAGAGTACTTTTTTT47940.21301374458523414No Hit
CCCATGTACTCTGCGTTGATACCAC36900.16395926523143806No Hit
CTGTAGGACGTGGAATATGGCAAGA31290.13903212490763406No Hit
GTCCTACAGTGGACATTTCTAAATT30500.13552188589590408No Hit
GTCCTAAAGTGTGTATTTCTCATTT28510.1266796382587615No Hit
CTTTAGGACGTGAAATATGGCGAGG26000.11552685355060675No Hit
GAGTACATGGGAAGCAGTGGTATCA24210.10757327401769962No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23450.10419633522160494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG551.9562955E-413.82195
TAGGACC6900.012.6695124
GTAGGAC19550.011.2273363
GTACCGT600.005875969311.0836066
TGTAGGA20150.010.9870912
ACCGTAT700.001492098910.8569288
GGCGAGG7450.010.7071519
AGGACGT18200.010.7034295
CTGTAGG20350.010.7028131
GGACGTG17550.010.447556
TGGCGAG17150.010.24419418
TCTACAC2053.6379788E-1210.19718553
GACGTGG9600.010.093557
TAGGACA6500.010.0868824
GTATTAA2750.010.0297981
GTCTTAC1909.822543E-1110.0115951
TAGGACG19750.09.8629754
ACGTGGA9650.09.8443648
AGGACCT13400.09.6443865
ATTTCTA11050.09.54067615
Produced by FastQC (version 0.11.5)