FastQCFastQC Report
Thu 26 May 2016
SRR1512512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70580.26780171896983224No Hit
CTGTAGGACGTGGAATATGGCAAGA61050.2316420365983035No Hit
GTCCTAAAGTGTGTATTTCTCATTT60350.2289860263506571No Hit
GTATCAACGCAGAGTACTTTTTTTT59970.22754419221622046No Hit
CTTTAGGACGTGAAATATGGCGAGG50920.19320577401450637No Hit
GTCCTACAGTGTGCATTTCTCATTT37140.1409203151394102No Hit
GGTATCAACGCAGAGTACTTTTTTT36030.13670864174671374No Hit
TATCAACGCAGAGTACTTTTTTTTT34980.13272462637524415No Hit
CTGAAGGACCTGGAATATGGCGAGA27640.10487446177849481No Hit
GTACATGGGAAGCAGTGGTATCAAC26390.1001315863362691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11900.013.4610154
GTATTAG2250.011.4431031
GGCGAGG17100.011.24406419
TTAGGAC23850.011.0880173
GTCCTAC36900.010.7882931
ACCGTGC803.9436948E-410.6362928
GGACGTG38550.010.5971966
GTGTAGG4300.010.5935721
AGGACGT38750.010.54250055
TAGGACA10900.010.5219674
AGGACCT24150.010.4792985
TGTAGGA41050.010.4134152
AATGTCC9500.010.350178
GTCCTAA17900.010.3452641
GTAGGAC40550.010.3316453
CCACCTT9750.010.31148113
GACGTGA21000.010.2909067
GGACCTG22950.010.2838266
CTAGGAC3600.010.2683383
CACCTTT9850.010.21010814
Produced by FastQC (version 0.11.5)