FastQCFastQC Report
Thu 26 May 2016
SRR1512512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80290.3046443754050416No Hit
GTCCTACAGTGGACATTTCTAAATT73170.27762895688612393No Hit
CTGTAGGACGTGGAATATGGCAAGA72900.2766044957906032No Hit
GTCCTAAAGTGTGTATTTCTCATTT66060.2506514813707441No Hit
CTTTAGGACGTGAAATATGGCGAGG62560.23737143013251213No Hit
TATCAACGCAGAGTACTTTTTTTTT54890.20826914641901523No Hit
GGTATCAACGCAGAGTACTTTTTTT51040.19366109005696003No Hit
GTCCTACAGTGTGCATTTCTCATTT38340.14547347556394685No Hit
CTGAAGGACCTGGAATATGGCGAGA32550.12350447651555739No Hit
CTGTAGGACCTGGAATATGGCGAGA29640.11246306248605595No Hit
GTACATGGGAAGCAGTGGTATCAAC29410.11159037340468642No Hit
TTTCTAAATTTTCCACCTTTTTCAG26850.10187696449900817No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26490.10051101637164717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12400.013.2572114
ACATTCG701.0950136E-412.21002818
TTGGACG550.00306391112.0938094
GGCGAGG18200.011.26972619
TGTAGGA40500.010.9804112
CATTCGT700.001497528710.85232819
TAGAAAT10200.010.8065124
GTAGGAC40850.010.700243
ATAGGAC4000.010.6900633
GACGGGA803.7754426E-410.6866137
GGACGTG40950.010.5778786
GCACCGC909.527928E-510.554686
GACGTGG20450.010.5443587
GTGTAGG3350.010.5062251
AGGACGT42050.010.4860695
CTGTAGG40500.010.4753761
ACCGTGG1659.313226E-1010.3627778
GTCCTAC34200.010.26337051
GGACCTG22950.010.1821626
GTACCGT750.002650439310.1324936
Produced by FastQC (version 0.11.5)