FastQCFastQC Report
Thu 26 May 2016
SRR1512511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992928
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71260.238094601674347No Hit
GTACATGGGAAGCAGTGGTATCAAC65650.21935041537918723No Hit
GTATCAACGCAGAGTACTTTTTTTT65050.21734568957221825No Hit
CTGTAGGACGTGGAATATGGCAAGA63020.2105630339253066No Hit
GTCCTAAAGTGTGTATTTCTCATTT61630.20591875247249514No Hit
CTTTAGGACGTGAAATATGGCGAGG49620.16579082423633312No Hit
CCCATGTACTCTGCGTTGATACCAC45830.15312763955564584No Hit
TATCAACGCAGAGTACTTTTTTTTT38200.12763420971035722No Hit
GGTATCAACGCAGAGTACTTTTTTT38180.1275673855167916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG3700.013.5694718
TAGGACC10850.011.7914644
GTGTAGG5650.011.7424481
GGCGAGG16350.011.35737719
CCAACGA2950.011.24050419
CCAACGT1701.2732926E-1111.14604719
ATGTCGA1752.1827873E-1110.84597217
TATGTCG1752.1827873E-1110.845242516
GGACGTG37450.010.8306786
ACCGTTT700.001552887410.8052848
GTCCTAT3300.010.8024151
AGGACGG1502.222805E-910.7404215
AGGACGT38700.010.6767535
AGGACCT22500.010.6140645
GTCCTAC33900.010.4279961
ACTGTTC9250.010.4256398
AAATGTC10250.010.2627287
TGTAGGA40400.010.2509412
GGACCTG21450.010.2500116
TTAGGAC21800.010.172393
Produced by FastQC (version 0.11.5)