FastQCFastQC Report
Thu 26 May 2016
SRR1512511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992928
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80040.26743042264965944No Hit
CTGTAGGACGTGGAATATGGCAAGA77790.25991270087352586No Hit
GTACATGGGAAGCAGTGGTATCAAC74790.24988907183868106No Hit
GTCCTACAGTGGACATTTCTAAATT74560.24912059361267627No Hit
GTCCTAAAGTGTGTATTTCTCATTT63140.2109639790867004No Hit
CTTTAGGACGTGAAATATGGCGAGG62530.2089258411829486No Hit
CCCATGTACTCTGCGTTGATACCAC55080.18403382907975066No Hit
TATCAACGCAGAGTACTTTTTTTTT52470.17531327181943568No Hit
GGTATCAACGCAGAGTACTTTTTTT51580.17233959520576506No Hit
CTGAAGGACCTGGAATATGGCGAGA35700.11928118551465322No Hit
GTCCTACAGTGTGCATTTCTCATTT33060.11046039196398978No Hit
GAGTACATGGGAAGCAGTGGTATCA32680.10919073228624278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11000.013.821894
TCCAACG4200.012.88899118
CGGTTTC3600.011.34533613
GGCGAGG17050.011.13979519
GCCGGTT3200.010.98328311
CCAACGT2350.010.91107519
AGGACCT22950.010.8482265
TAGGACT5400.010.7343624
TTAGGAC24200.010.6016643
AGGACGT41450.010.4768765
ACCTTTT10800.010.46532815
GACGTGA21950.010.3858637
GGACCTG22850.010.3512696
GGACGTG40850.010.3245546
ACTGTTC9850.010.3184278
TGGCGAG39000.010.30075618
CACCTTT10900.010.28217914
TGTAGGA40750.010.2600782
CTAGGAC3800.010.2524083
CCACCTT10700.010.20858413
Produced by FastQC (version 0.11.5)