FastQCFastQC Report
Thu 26 May 2016
SRR1512510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538650
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62250.24520906781163218No Hit
GTCCTAAAGTGTGTATTTCTCATTT51560.20310007287337759No Hit
GTCCTACAGTGGACATTTCTAAATT51510.20290311779883008No Hit
GTACATGGGAAGCAGTGGTATCAAC50030.1970732475922242No Hit
CTGTAGGACGTGGAATATGGCAAGA48920.19270084493726983No Hit
CTTTAGGACGTGAAATATGGCGAGG43590.17170543399050675No Hit
GGTATCAACGCAGAGTACTTTTTTT37260.1467709215527938No Hit
TATCAACGCAGAGTACTTTTTTTTT35850.14121678845055444No Hit
CCCATGTACTCTGCGTTGATACCAC34360.13534752722903906No Hit
GTCCTACAGTGTGCATTTCTCATTT27550.10852224607567013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG604.1377955E-412.6505516
GTGTAGG4200.011.3169341
TAGGACC9750.010.9847124
CCAACGA1303.4153345E-810.92896719
GTCCTAA15650.010.6299621
TCCAACG2050.010.62539518
GTCCTAG3300.010.5024571
GGCGAGG14550.010.41569519
AGGACGT30450.010.2405555
TTAGGAC19950.010.1668343
GTATTAG2153.6379788E-1210.1326031
GGACGTG30550.010.051916
AGGACCT20600.010.0300735
GCGTGCG1054.2232037E-59.9271299
GTCCTAC25900.09.8640831
GACGTGG14500.09.8047867
GTATTAA3450.09.7588031
GGACCTG19850.09.7405736
AAATGTC8400.09.7036267
AATCCCG2650.09.65048519
Produced by FastQC (version 0.11.5)