FastQCFastQC Report
Thu 26 May 2016
SRR1512510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538650
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78230.3081559096370118No Hit
CTGTAGGACGTGGAATATGGCAAGA58420.230122309101294No Hit
GTCCTACAGTGGACATTTCTAAATT58320.22972839895219901No Hit
GTACATGGGAAGCAGTGGTATCAAC57260.2255529513717921No Hit
GTCCTAAAGTGTGTATTTCTCATTT56390.2221259330746657No Hit
CTTTAGGACGTGAAATATGGCGAGG52330.20613318102140904No Hit
TATCAACGCAGAGTACTTTTTTTTT51990.20479388651448602No Hit
GGTATCAACGCAGAGTACTTTTTTT50910.20053965690426012No Hit
CCCATGTACTCTGCGTTGATACCAC43460.17119335079668327No Hit
GTCCTACAGTGTGCATTTCTCATTT30220.11903964705650641No Hit
CTGAAGGACCTGGAATATGGCGAGA28510.11230378350698204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7700.013.8206334
AGACCCG550.003064387512.093535
AGTCGAG658.0318E-411.69138710
AGGACGT32300.011.1494765
GGACGTG32500.010.9024326
ACGACTG700.00148927110.8594965
TTAGGAC19350.010.8029493
CCCGTTT1355.698712E-810.55472412
GTAGGAC31450.010.5137883
ACTGTTC6700.010.4917118
GGCGAGG15500.010.47734219
TGTAGGA32550.010.3918072
GACGTGG16650.010.3265127
GACGTGA18150.010.3104837
TCCAACG1856.002665E-1110.26784218
GTCCTAT2800.010.1908191
TGGCGAG31050.010.15540818
AGTCGTC2250.010.1325358
TAGGACG36050.010.1210744
CTGTAGG33150.010.0709281
Produced by FastQC (version 0.11.5)