FastQCFastQC Report
Thu 26 May 2016
SRR1512509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190590
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51010.2328596405534582No Hit
GTACATGGGAAGCAGTGGTATCAAC48290.22044289438005288No Hit
CTGTAGGACGTGGAATATGGCAAGA47970.2189821007125934No Hit
GTCCTACAGTGGACATTTCTAAATT46820.213732373470161No Hit
GTCCTAAAGTGTGTATTTCTCATTT44490.20309596957897189No Hit
CTTTAGGACGTGAAATATGGCGAGG37730.17223670335389096No Hit
CCCATGTACTCTGCGTTGATACCAC34110.1557114749907559No Hit
GGTATCAACGCAGAGTACTTTTTTT33030.15078129636308027No Hit
TATCAACGCAGAGTACTTTTTTTTT31320.1429751802025938No Hit
CTGAAGGACCTGGAATATGGCGAGA22260.10161645949264811No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGT350.0022052216.247154
CTTACGG456.886535E-414.74278453
TAGGACC6450.011.314234
TGTCGAG1451.2823875E-911.10340518
TACACCG700.001521612910.8314335
AGGACGT30450.010.7380595
AAATGTC6650.010.6889157
GTCCTAT3750.010.5726461
GGACGTG29700.010.53056056
GACGTGG16600.010.3909997
GTCGAGG1201.5838796E-610.2609419
CTAGACT2500.010.2357044
GGCGAGG12000.010.1820119
AGGCGTG1401.0034455E-710.1544697
GATATAC3250.010.0643461
TCCAACG1606.49743E-910.0624618
CCTACAC2450.010.057763
CATTTCG856.6824513E-410.04445314
CCGTGCA951.6874414E-49.9744899
GTAGGAC28000.09.9175313
Produced by FastQC (version 0.11.5)