FastQCFastQC Report
Thu 26 May 2016
SRR1512509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190590
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72890.3327414075659982No Hit
GTACATGGGAAGCAGTGGTATCAAC56800.25929087597405265No Hit
CTGTAGGACGTGGAATATGGCAAGA55570.25367595031475537No Hit
GTCCTACAGTGGACATTTCTAAATT52050.23760721997270143No Hit
GTCCTAAAGTGTGTATTTCTCATTT49450.22573827142459338No Hit
TATCAACGCAGAGTACTTTTTTTTT49260.22487092518453933No Hit
GGTATCAACGCAGAGTACTTTTTTT46810.2136867236680529No Hit
CTTTAGGACGTGAAATATGGCGAGG46500.21227157980270153No Hit
CCCATGTACTCTGCGTTGATACCAC41100.18762068666432333No Hit
CTGAAGGACCTGGAATATGGCGAGA25670.11718304201151289No Hit
GAGTACATGGGAAGCAGTGGTATCA23440.10700313614140483No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22930.10467499623389133No Hit
GTCCTACAGTGTGCATTTCTCATTT22880.10444674722335079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7150.012.2256284
CGTGCGC658.030903E-411.69140810
TGGACCG907.4517793E-611.6128695
ACCTTTT7250.011.52909515
GTCCTAT2700.011.2774481
TTTAGAA9050.011.2334892
CAAACGT600.005870338511.08501154
AATGTCC7950.010.6341598
GTCCTAA14450.010.6018731
AGGACGT28750.010.50941855
GACGTGG16550.010.4460887
ATTTAGA8350.010.3700181
CCACCTT7900.010.34074113
GGACGTG28550.010.2809366
GGCGAGG11450.010.20189319
AAATGTC8200.010.1941057
TGTAGGA30850.010.1633822
GTAGGAC29700.09.9810813
GTAGAAC5450.09.93705753
GTATTAG1151.0072439E-59.9290561
Produced by FastQC (version 0.11.5)