FastQCFastQC Report
Thu 26 May 2016
SRR1512508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689741
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76820.285603706825304No Hit
CTGTAGGACGTGGAATATGGCAAGA70440.2618839509082845No Hit
GTCCTAAAGTGTGTATTTCTCATTT69420.2580917642256262No Hit
CTTTAGGACGTGAAATATGGCGAGG58520.21756741634231697No Hit
GTACATGGGAAGCAGTGGTATCAAC56600.2104291825867249No Hit
GTATCAACGCAGAGTACTTTTTTTT47060.17496108361362675No Hit
GTCCTACAGTGTGCATTTCTCATTT43090.1602012981919077No Hit
CCCATGTACTCTGCGTTGATACCAC40460.15042340507877897No Hit
CTGAAGGACCTGGAATATGGCGAGA29920.11123747602464326No Hit
CTGTAGGACCTGGAATATGGCGAGA28660.10655301012253596No Hit
GGTATCAACGCAGAGTACTTTTTTT28370.10547483939903507No Hit
GTCCTTCAGTGTGCATTTCTCATTT28120.10454538187877568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13250.013.0888884
TCCAACG2100.012.62835718
TACACCG1001.4903708E-712.3200055
ACACCGT907.670902E-611.58291156
GGCGAGG18350.011.56424519
ACCTTTT11550.011.3353615
CACCTTT11600.011.20471414
AGGACCT26200.010.9961295
AAATGTC11550.010.9948777
TAGAAAT12900.010.94621854
GGACCTG25850.010.7417396
ATTTAGA12100.010.7231281
TAGGACA12250.010.6760494
CCAACGA1605.7298166E-1010.65756319
CCACCTT11850.010.64330913
AATGTCC12500.010.5929918
GTCCTAC37600.010.5631181
GTGTAGG6250.010.4592251
TTTAGAA14000.010.4252032
TGTAGGA45750.010.4200252
Produced by FastQC (version 0.11.5)