FastQCFastQC Report
Thu 26 May 2016
SRR1512508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689741
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA83470.31032727686420364No Hit
GTCCTACAGTGGACATTTCTAAATT82960.3084311835228745No Hit
GTCCTAAAGTGTGTATTTCTCATTT76200.28329865217506073No Hit
CTTTAGGACGTGAAATATGGCGAGG67710.251734274787052No Hit
GTACATGGGAAGCAGTGGTATCAAC65210.24243969958445813No Hit
GTATCAACGCAGAGTACTTTTTTTT60980.22671327834166932No Hit
CCCATGTACTCTGCGTTGATACCAC48540.1804634721335623No Hit
GTCCTACAGTGTGCATTTCTCATTT46830.17410598269498812No Hit
TATCAACGCAGAGTACTTTTTTTTT40380.15012597867229596No Hit
GGTATCAACGCAGAGTACTTTTTTT39180.1456645825750509No Hit
CTGAAGGACCTGGAATATGGCGAGA37840.1406826902664606No Hit
CTGTAGGACCTGGAATATGGCGAGA35350.13142529336467712No Hit
ATTTAGAAATGTCCACTGTAGGACG31250.1161821900324232No Hit
GTCCTTCAGTGTGCATTTCTCATTT29890.11112594112221214No Hit
GAATATGGCAAGAAAACTGAAAATC29660.1102708402035735No Hit
GAGTACATGGGAAGCAGTGGTATCA28600.10632994031767372No Hit
TTTCTAAATTTTCCACCTTTTTCAG28390.10554919600065582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTTA400.00528156314.2495397
TAGGACC13850.012.8994474
CCAACGA1850.012.8326519
GGCGAGG18700.012.13680619
TCCAACG2850.011.99537418
AGGACCT27200.011.5296525
ATACGGC1001.9370564E-611.39708915
GGACCTG26650.011.0148586
AATGTCC11650.010.9266868
CCACCTT11500.010.90277913
CACCTTT11500.010.90156214
AGGACGT45200.010.8488175
ACCTTTT11850.010.82002115
GACGTGG23450.010.8162817
TGTAGGA46950.010.7670982
TGGCGAG45000.010.76251618
TGTCGAG1605.493348E-1010.68337918
GGACGTG44550.010.6193846
CTGTAGG47350.010.5657581
GCGCCAC1803.6379788E-1110.55403713
Produced by FastQC (version 0.11.5)