FastQCFastQC Report
Thu 26 May 2016
SRR1512507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940175
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51240.26409988789671035No Hit
GTCCTACAGTGGACATTTCTAAATT50000.2577087118430038No Hit
CTGTAGGACGTGGAATATGGCAAGA45500.2345149277771335No Hit
GTCCTAAAGTGTGTATTTCTCATTT44270.22817529346579563No Hit
CTTTAGGACGTGAAATATGGCGAGG37160.19152911464172048No Hit
GGTATCAACGCAGAGTACTTTTTTT31240.16101640315950883No Hit
TATCAACGCAGAGTACTTTTTTTTT30140.15534681149896273No Hit
GTCCTACAGTGTGCATTTCTCATTT23690.12210238767121522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGT456.886021E-414.7427876
TAGGACC8600.013.8856494
TCCAACG1351.8189894E-1213.32801218
GTGTAGG3750.013.2122351
CCAACGA1052.0765583E-812.63013219
GTCTTAC2150.011.98319151
GTTCTAA2000.011.3955541
GGCGAGG11600.011.35078819
AATGTCC6350.011.0181148
CCTAGAC1303.3829565E-810.9355853
GTTTAGG2850.010.7784031
AGGACGT28850.010.7094185
GTAGGAC29050.010.7009373
TGTAGGA29950.010.6644492
TGTCCAC7250.010.59030810
GGACGTG28500.010.5416476
TTAGGAC17650.010.5245973
GAAATGT6150.010.4791946
GTCCTAC24700.010.4708311
AGGACCT18800.010.4353395
Produced by FastQC (version 0.11.5)