FastQCFastQC Report
Thu 26 May 2016
SRR1512507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940175
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66510.34280412849356373No Hit
CTGTAGGACGTGGAATATGGCAAGA55990.2885822155217957No Hit
GTCCTACAGTGGACATTTCTAAATT54790.2823972064375636No Hit
GTCCTAAAGTGTGTATTTCTCATTT47860.24667877897612328No Hit
CTTTAGGACGTGAAATATGGCGAGG45830.23621580527529734No Hit
TATCAACGCAGAGTACTTTTTTTTT43870.22611362377105157No Hit
GGTATCAACGCAGAGTACTTTTTTT43340.22338191142551575No Hit
GTCCTACAGTGTGCATTTCTCATTT25740.1326684448567784No Hit
CTGAAGGACCTGGAATATGGCGAGA24970.1286997306943961No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21160.10906232685195924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC1051.371518E-913.57133711
AAAGGCG905.3958865E-712.6688685
TAGGACC9300.012.668544
GTTCTAA1251.3860699E-912.1740431
AATCCCG2500.011.77475919
GGTATCA15950.011.2700531
GCGCCAC1104.9720984E-711.22690813
GACCGTG855.3245712E-511.1763947
GGCGAGG11850.011.13850219
CACCTTT6050.010.99024514
TCTACAC2250.010.9788353
CGAAATC2250.010.9774213
CCACCTT5900.010.94879613
TTATACC1658.0035534E-1110.9410124
ACGAAAT2250.010.55548312
GTCCTAC26250.010.5073591
GTCTTAC1855.820766E-1110.2821311
AGGACCT19450.010.2588515
TCCTACA28450.010.2523062
GTCTAAT1303.7816062E-710.2425831
Produced by FastQC (version 0.11.5)