FastQCFastQC Report
Thu 26 May 2016
SRR1512506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731050
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79110.2896688086999506No Hit
CTGTAGGACGTGGAATATGGCAAGA72450.265282583621684No Hit
GTCCTAAAGTGTGTATTTCTCATTT68900.2522839201039893No Hit
CTTTAGGACGTGAAATATGGCGAGG57810.21167682759378262No Hit
GTATCAACGCAGAGTACTTTTTTTT55010.20142436059391078No Hit
GTCCTACAGTGTGCATTTCTCATTT40620.1487340034052837No Hit
GGTATCAACGCAGAGTACTTTTTTT33530.12277329232346533No Hit
TATCAACGCAGAGTACTTTTTTTTT32670.11962432031636183No Hit
CTGAAGGACCTGGAATATGGCGAGA30020.10992109262005455No Hit
GTACATGGGAAGCAGTGGTATCAAC27690.10138957543801834No Hit
CTGTAGGACCTGGAATATGGCGAGA27460.10054740850588603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG400.005363004314.2167147
TAGGACC13750.012.820894
CCAACGA1001.4997022E-712.31412719
AGGACCG854.0508985E-612.2654015
GTGTAGG5250.012.073061
TCGCATT752.1328073E-411.3662618
GTCCTAA20350.011.1933511
GGCGAGG17150.011.15700219
AGGACCT24850.011.0606225
AGTACCG600.005980768711.0574445
TGTAGGA43950.011.0410672
GGACCTG24250.010.943456
TCCAACG1658.54925E-1110.90701718
GCACCGT1053.5672656E-610.8317826
GGACGTA1053.5672656E-610.8317826
GTATAGG2850.010.7724271
AATGTCC10450.010.6762858
GTAGGAC42750.010.641753
GTCCTAC35050.010.5959511
AAATGTC10250.010.5411737
Produced by FastQC (version 0.11.5)