FastQCFastQC Report
Thu 26 May 2016
SRR1512506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2731050
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA86650.3172772376924626No Hit
GTCCTACAGTGGACATTTCTAAATT83860.3070613866461617No Hit
GTCCTAAAGTGTGTATTTCTCATTT73620.26956665018948756No Hit
GTATCAACGCAGAGTACTTTTTTTT72570.26572197506453565No Hit
CTTTAGGACGTGAAATATGGCGAGG69320.25382179015397005No Hit
TATCAACGCAGAGTACTTTTTTTTT46400.16989802456930486No Hit
GGTATCAACGCAGAGTACTTTTTTT45720.16740813972647883No Hit
GTCCTACAGTGTGCATTTCTCATTT43160.15803445561231028No Hit
CTGAAGGACCTGGAATATGGCGAGA38560.1411911169696637No Hit
CTGTAGGACCTGGAATATGGCGAGA35390.12958385968766592No Hit
GTACATGGGAAGCAGTGGTATCAAC33220.12163819776276523No Hit
ATTTAGAAATGTCCACTGTAGGACG29660.10860291829149962No Hit
GTCCTTCAGTGTGCATTTCTCATTT28160.10311052525585397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12000.012.9076014
GTAGACG550.003063358112.0941274
GGACCGT658.0311176E-411.6915646
CTAGGAC4200.011.3123263
GGCGAGG15950.011.25283219
TCGAACT1701.2732926E-1111.1722819
TCCAACG1803.6379788E-1211.07958418
GGACCTG23950.010.86778456
TGGACCG700.001488492810.8602315
TAGTACG700.001488720310.8600324
AGGACGT41800.010.7530585
GGACGTG40650.010.7262546
TGTAGGA41750.010.6972612
GTCCTAC40150.010.6382371
AGGACCT25050.010.6217835
GACGTGG22650.010.568677
TAGAAAT10800.010.5583654
GTAGGAC41600.010.5302533
CTGTAGG42050.010.451651
TCTACAC3550.010.4392063
Produced by FastQC (version 0.11.5)