FastQCFastQC Report
Thu 26 May 2016
SRR1512505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278490
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT93750.28595481456402183No Hit
CTGTAGGACGTGGAATATGGCAAGA83700.2553004584427587No Hit
GTCCTAAAGTGTGTATTTCTCATTT81790.24947460568737434No Hit
CTTTAGGACGTGAAATATGGCGAGG70110.21384844852355808No Hit
GTCCTACAGTGTGCATTTCTCATTT60650.18499370136861787No Hit
GTATCAACGCAGAGTACTTTTTTTT59100.18026591510115936No Hit
CTGTAGGACCTGGAATATGGCGAGA39680.12103132844693747No Hit
GGTATCAACGCAGAGTACTTTTTTT35920.10956263401748975No Hit
CTGAAGGACCTGGAATATGGCGAGA35680.10883058969220587No Hit
TATCAACGCAGAGTACTTTTTTTTT34320.10468233851559712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT250.00611700118.9548176
GACGACG350.002240456616.2089798
TAGGACC18050.013.2316184
TCCAACG2900.012.73806418
TAGGCCG854.053516E-612.2648825
CCAACGA2250.012.2112119
GTCGTGC550.003180191212.0339398
GGCGAGG19600.011.84276819
GCAACGT600.00595108311.06494813
CCACCTT12700.011.05250213
TGTAGGA53150.010.9306722
CCCGTTT2200.010.77275512
AGGACCT32900.010.7449045
GTCCTAC51000.010.6764411
TAGACCG803.8569813E-410.6620855
ACCTTTT12950.010.62430915
GTAGGAC53650.010.59915353
CACCTTT13800.010.58873514
GGACCTG32300.010.5337156
GTCCTAT5000.010.4939711
Produced by FastQC (version 0.11.5)