FastQCFastQC Report
Thu 26 May 2016
SRR1512505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278490
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT101400.309288727432446No Hit
CTGTAGGACGTGGAATATGGCAAGA100570.3067570741408392No Hit
GTCCTAAAGTGTGTATTTCTCATTT89410.27271701301513807No Hit
CTTTAGGACGTGAAATATGGCGAGG82900.2528603106918124No Hit
GTATCAACGCAGAGTACTTTTTTTT78360.23901247220519203No Hit
GTCCTACAGTGTGCATTTCTCATTT66760.2036303298164704No Hit
TATCAACGCAGAGTACTTTTTTTTT52200.15921964074924738No Hit
GGTATCAACGCAGAGTACTTTTTTT49750.15174668826197427No Hit
CTGTAGGACCTGGAATATGGCGAGA48470.14784245186046013No Hit
CTGAAGGACCTGGAATATGGCGAGA44190.13478766139289736No Hit
ATTTAGAAATGTCCACTGTAGGACG36910.11258231685928584No Hit
GTACATGGGAAGCAGTGGTATCAAC35490.1082510546013561No Hit
TTTCTAAATTTTCCACCTTTTTCAG34150.10416380711852102No Hit
GAATATGGCAAGAAAACTGAAAATC33230.10135763720493275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT350.002168143916.2888014
TCCAACG3250.013.44275418
TAGGACC17350.013.0889374
CCAACGA2350.012.12403819
TAAACCG658.014834E-411.6945255
CGGTTTC2250.011.39868213
TGTAGGA54700.011.3081862
GTAGGAC54900.010.9727683
ACCTTTT12550.010.8981415
TAAGCCG700.001489809510.8592015
GGCGAGG22800.010.83009819
AGGACGT52500.010.8230045
AAGGCGT1951.8189894E-1210.716716
GCCGGTT2400.010.68659111
AAATGTC13100.010.6592357
CTGTAGG55250.010.6417371
GGACGTG51700.010.6196626
GGACCTG32350.010.600016
AGGACCT33100.010.5926425
GACGTGG26400.010.5065217
Produced by FastQC (version 0.11.5)