FastQCFastQC Report
Thu 26 May 2016
SRR1512504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197663
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39090.32638563602616094No Hit
GGTATCAACGCAGAGTACTTTTTTT24010.20047375597309092No Hit
TATCAACGCAGAGTACTTTTTTTTT22530.18811635660448722No Hit
GTCCTAAAGTGTGTATTTCTCATTT17730.148038304598205No Hit
GTCCTACAGTGGACATTTCTAAATT17380.14511594663941357No Hit
CTGTAGGACGTGGAATATGGCAAGA16980.1417761089722234No Hit
GTACATGGGAAGCAGTGGTATCAAC15280.12758179888666513No Hit
CTTTAGGACGTGAAATATGGCGAGG15230.12716431917826634No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12200.10186504884930067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG602.654808E-514.19941318
CTCCTAG707.3614545E-512.7385671
CCAACGA604.2078985E-412.62434719
TAGGACC3450.012.3623454
ATAAGAC907.6530905E-611.5839743
CCAGTAC1501.8371793E-1011.3733583
GTCTTAG1759.094947E-1211.3231721
GTCTAAC750.001866484510.5682941
GTCCTAT1603.085006E-910.527011
GTGTAGG2001.0913936E-1110.4031641
GGACGTG11600.010.2948486
TGTAGGA12500.010.1601992
AGGACGT12100.010.1044285
GGCGAGG5300.010.00419919
GTCCTAC10050.09.95706751
GACGTGG6000.09.9516887
GTAGGAC12100.09.947773
GTCCTAG1302.4762903E-69.9077751
CCCTTAG800.00318942969.9077751
GTATAGG1405.9539707E-79.9077751
Produced by FastQC (version 0.11.5)