FastQCFastQC Report
Thu 26 May 2016
SRR1512504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197663
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50860.42466035938323216No Hit
GGTATCAACGCAGAGTACTTTTTTT35030.29248628370418056No Hit
TATCAACGCAGAGTACTTTTTTTTT33220.277373518260145No Hit
GTCCTAAAGTGTGTATTTCTCATTT19830.1655724523509535No Hit
CTGTAGGACGTGGAATATGGCAAGA19770.16507147670087494No Hit
GTCCTACAGTGGACATTTCTAAATT18960.1583083054248148No Hit
CTTTAGGACGTGAAATATGGCGAGG18300.15279757327395102No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17550.14653537764796942No Hit
GTACATGGGAAGCAGTGGTATCAAC17280.14428098722261604No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14700.12273903426923934No Hit
CCCATGTACTCTGCGTTGATACCAC13020.10871171606704057No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11980.10002813813234608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4250.013.4145964
AGGACCT7100.011.375645
GGACCTG6900.011.1517336
CCGTGTC600.00587422611.0833589
GACGTGA7100.010.83767
CTAGCAC803.7634172E-410.689313
GGCGAGG6150.010.65366519
AATGTCC3850.010.6099698
TGGGTTG1553.6434358E-910.4215555
TTAGGAC8700.010.37531953
CCACCTT3250.010.22908313
ACGAAAT856.590452E-410.058845512
GAAATGT4250.010.0584266
ACTTATC951.643762E-49.9996048
TGGCGAG12900.09.93814618
ATGTCCA3650.09.8904329
GTCCTAC10400.09.7879621
TTTAGGA9350.09.7556632
TTTAGAA5650.09.7538632
AGGACGT12100.09.7375935