##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197663 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56274344285496 33.0 31.0 34.0 30.0 34.0 2 31.83944815862225 33.0 31.0 34.0 30.0 34.0 3 32.002882279906785 34.0 31.0 34.0 30.0 34.0 4 35.486592639164776 37.0 35.0 37.0 33.0 37.0 5 34.9603085342037 37.0 35.0 37.0 32.0 37.0 6 35.19106877310228 37.0 35.0 37.0 33.0 37.0 7 35.148227840385815 37.0 35.0 37.0 32.0 37.0 8 35.130827286139755 37.0 35.0 37.0 32.0 37.0 9 36.71982268801825 39.0 37.0 39.0 33.0 39.0 10 36.67206217441801 39.0 37.0 39.0 33.0 39.0 11 36.70957022133939 39.0 37.0 39.0 33.0 39.0 12 36.633479534727215 39.0 37.0 39.0 33.0 39.0 13 36.5979478367454 39.0 37.0 39.0 32.0 39.0 14 37.88065090096296 40.0 38.0 41.0 33.0 41.0 15 37.87030742370767 40.0 38.0 41.0 33.0 41.0 16 37.850921335968465 40.0 38.0 41.0 33.0 41.0 17 37.83256892798725 40.0 38.0 41.0 33.0 41.0 18 37.7796625595013 40.0 38.0 41.0 32.0 41.0 19 37.81922627650683 40.0 38.0 41.0 32.0 41.0 20 37.80450510702927 40.0 38.0 41.0 32.0 41.0 21 37.74451410789179 40.0 38.0 41.0 32.0 41.0 22 37.67805718303062 40.0 38.0 41.0 32.0 41.0 23 37.57597838457062 40.0 38.0 41.0 32.0 41.0 24 37.53901723606724 40.0 38.0 41.0 32.0 41.0 25 37.47830733687189 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11932830596789756 1101 2 0.28367942949576275 1101 3 0.24519679998556398 1101 4 0.2247560370671451 1101 5 0.05735033024586045 1101 6 0.24267353109483736 1101 7 0.2348587092710659 1101 8 0.25283065106074076 1101 9 0.500819546318489 1101 10 0.44752624936212015 1101 11 0.4277182390705647 1101 12 0.37402121214112327 1101 13 0.3696664140936363 1101 14 0.6306967839637281 1101 15 0.5244754566120662 1101 16 0.6106918801656818 1101 17 0.5140818768868698 1101 18 0.35685432869787803 1101 19 0.4527272667020341 1101 20 0.49940499718402975 1101 21 0.43141273544075176 1101 22 0.4500573767763143 1101 23 0.31568359000635127 1101 24 0.43145774778482604 1101 25 0.23265758328795272 1102 1 -0.030760835969068978 1102 2 0.11556845431957186 1102 3 0.19285924221322404 1102 4 0.23404143765689867 1102 5 -0.15094771229131254 1102 6 0.05757330523373838 1102 7 0.27406155553912726 1102 8 0.25017207774999406 1102 9 0.2619544460403489 1102 10 0.42441469907665663 1102 11 0.2522889984239356 1102 12 0.29069180000735884 1102 13 0.2922671627813429 1102 14 0.43696991904121063 1102 15 0.4500210261793711 1102 16 0.47597645468938765 1102 17 0.5710765126858561 1102 18 0.6409844497845256 1102 19 0.40807526009350426 1102 20 0.6251447968368709 1102 21 0.38990160969049015 1102 22 0.462916297243936 1102 23 0.4601131338924134 1102 24 0.5463332104863738 1102 25 0.40823713658489424 1103 1 -0.1894800059238122 1103 2 -0.11809992857764229 1103 3 -0.08645128959126325 1103 4 0.007368491175292036 1103 5 -0.3100559029101788 1103 6 -0.14340725382110975 1103 7 -0.016821237641849507 1103 8 0.06774156238903828 1103 9 0.17034417791242618 1103 10 0.10792526694594784 1103 11 0.1164621498191778 1103 12 0.08018559396133895 1103 13 0.22668723196019158 1103 14 0.13794858658553721 1103 15 0.28162047486710406 1103 16 0.12915349881328808 1103 17 0.19383365408383213 1103 18 0.08395162451091664 1103 19 0.22597702008099674 1103 20 0.30266242886745687 1103 21 0.04025715061836621 1103 22 -0.07549586794294072 1103 23 0.1181242484082432 1103 24 0.09202075564618895 1103 25 0.17260599626886375 1104 1 -0.12174312260352949 1104 2 -0.020507455048264234 1104 3 -0.05434294598275358 1104 4 0.053356008524261256 1104 5 -0.43712840530430697 1104 6 -0.242064944715068 1104 7 0.12253353444589266 1104 8 -0.06825897360030808 1104 9 0.04460611689077609 1104 10 -0.13423127882398234 1104 11 0.019626330978660178 1104 12 0.09059927693347447 1104 13 -0.016946972519747305 1104 14 0.029003834056496203 1104 15 0.11020222983884054 1104 16 0.031940274628361465 1104 17 0.12656760544403056 1104 18 0.22823295007650302 1104 19 0.09517410557744199 1104 20 0.10843882050356513 1104 21 0.22989104284762618 1104 22 0.35153090324984504 1104 23 0.21568537393670795 1104 24 0.3338410787395816 1104 25 0.19160042615936135 1105 1 0.11180168310622562 1105 2 0.2922423871566622 1105 3 0.2953875885784143 1105 4 0.2805404588309486 1105 5 -0.023575831221542387 1105 6 0.255208032675732 1105 7 0.33041843305243646 1105 8 0.40867866902216576 1105 9 0.42568527337493833 1105 10 0.5125990631462756 1105 11 0.5835778433837788 1105 12 0.6044447834152109 1105 13 0.6485112805673694 1105 14 0.7391302319590309 1105 15 0.8202962595204966 1105 16 0.660320730696597 1105 17 0.6970979587431572 1105 18 0.7096535912909587 1105 19 0.47966810936452475 1105 20 0.45808034245731477 1105 21 0.5072138672985815 1105 22 0.6008228484180762 1105 23 0.5782580001323083 1105 24 0.6226222533823176 1105 25 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0.20297784253248352 1107 13 0.3296726494187041 1107 14 0.26636929787142805 1107 15 0.28728771000008635 1107 16 0.26733311027840756 1107 17 0.38763934904712016 1107 18 0.43538776075719454 1107 19 0.3702638748800524 1107 20 0.5348927652299338 1107 21 0.5035628752484271 1107 22 0.4661900625451594 1107 23 0.5436303900180377 1107 24 0.6000404778829349 1107 25 0.6114310510055319 1108 1 0.08274860316479149 1108 2 0.04834674602478373 1108 3 0.1670943468831112 1108 4 0.21637946486078619 1108 5 -0.09049271520356683 1108 6 0.06607023880005869 1108 7 0.24120446162745424 1108 8 0.1599092895223606 1108 9 0.25347464338847914 1108 10 0.17305279137801932 1108 11 0.4450803096241245 1108 12 0.2086689816293159 1108 13 0.41499303388834363 1108 14 0.4788036121237482 1108 15 0.579205252033347 1108 16 0.47619915657721634 1108 17 0.5616887715682353 1108 18 0.41531891375821317 1108 19 0.5088392988778949 1108 20 0.5954055293547711 1108 21 0.5768341481493948 1108 22 0.49457422714423416 1108 23 0.49488889022228477 1108 24 0.6480051877365156 1108 25 0.6981093399631106 1109 1 -0.07841230120187248 1109 2 0.04702427041219437 1109 3 -0.05738861384702432 1109 4 0.019826452710283604 1109 5 -0.3169590924339474 1109 6 -0.0508438501674604 1109 7 -0.069546427792325 1109 8 -0.09371682934129666 1109 9 -0.13623344402198967 1109 10 -0.07334276481887514 1109 11 -0.035649967703818675 1109 12 -0.22032975527879728 1109 13 -0.10903273152337078 1109 14 -0.15550011070151726 1109 15 -0.28975802091267155 1109 16 -0.2434959911190262 1109 17 -0.3050743342719784 1109 18 -0.4216299978271394 1109 19 -0.3768499947704811 1109 20 -0.3931316297158247 1109 21 -0.30128490791062035 1109 22 -0.2758488759762088 1109 23 -0.09982191993209 1109 24 -0.05767896328104882 1109 25 -0.283798219763554 1110 1 0.05501690767655987 1110 2 -0.03953411887864888 1110 3 -0.03923082764796959 1110 4 0.06316554580217115 1110 5 -0.18283506157654728 1110 6 0.1355116260052256 1110 7 0.16267129222180188 1110 8 0.15193183375390618 1110 9 0.2617831758002893 1110 10 0.13702628377131276 1110 11 0.20609845083458112 1110 12 0.17007976184274298 1110 13 0.30898825803319596 1110 14 0.20759457391034886 1110 15 0.20138356931692414 1110 16 0.11234413593413706 1110 17 0.19349694516454718 1110 18 0.0413490542599817 1110 19 -0.0066022947282107225 1110 20 0.14278319355238267 1110 21 0.22266505806135228 1110 22 0.17219207402456504 1110 23 0.34550591567462163 1110 24 0.14842246361403255 1110 25 0.28672364402509487 1111 1 -0.31642703064950695 1111 2 -0.2211347218701114 1111 3 -0.1571393695919845 1111 4 -0.18219835911138915 1111 5 -0.5247608057445845 1111 6 -0.2640159001635567 1111 7 -0.13364778423542845 1111 8 -0.20609978631797077 1111 9 -0.27772016110052533 1111 10 -0.19347427310392362 1111 11 -0.1348750584365206 1111 12 -0.34575039556152376 1111 13 -0.12607921958303336 1111 14 -0.037938460102552085 1111 15 -0.12463261922015079 1111 16 -0.08116511341545163 1111 17 -0.12557516147706593 1111 18 -0.14082256337556487 1111 19 -0.24506344833005755 1111 20 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2314 16 -1.4912595660089067 2314 17 -1.2482081492369659 2314 18 -1.0293829019878018 2314 19 -1.0963684645314018 2314 20 -1.1122627150604671 2314 21 -1.1112193350842574 2314 22 -1.134984383914329 2314 23 -1.004494384506522 2314 24 -0.9297769079703784 2314 25 -0.988624334145733 2315 1 -0.9580384062672351 2315 2 -0.7774535165056022 2315 3 -0.5903295158039086 2315 4 -0.4146834707934133 2315 5 -0.1342508317500375 2315 6 -0.2830014518169861 2315 7 -0.39232003303925467 2315 8 -0.8185124794929095 2315 9 -0.8065540011866332 2315 10 -0.6627779201525783 2315 11 -0.6587479263429685 2315 12 -0.6748082478725479 2315 13 -0.7615707697751262 2315 14 -0.8132967225297776 2315 15 -0.7498324723011294 2315 16 -0.8382571970728705 2315 17 -0.9106019918759429 2315 18 -1.0271169631681474 2315 19 -0.879381414090858 2315 20 -0.6971090151194588 2315 21 -0.7381446808271903 2315 22 -0.7145871891840372 2315 23 -0.5518403334386264 2315 24 -0.4882278297954912 2315 25 -0.4591999013326955 2316 1 -0.6518229261639945 2316 2 -0.6483126499410687 2316 3 -0.7101309447068189 2316 4 -0.7436055583662693 2316 5 -0.4902462950441375 2316 6 -0.740328848661683 2316 7 -1.0494320076853967 2316 8 -0.9766275058835987 2316 9 -1.1216586890741098 2316 10 -1.126243213219496 2316 11 -1.124006586134918 2316 12 -1.1475605287085742 2316 13 -1.0374274128896914 2316 14 -1.2518495754472738 2316 15 -1.33037683504962 2316 16 -1.4132905447145347 2316 17 -1.5615735527050205 2316 18 -1.4600778183447645 2316 19 -1.6303140780796426 2316 20 -1.5626560234405247 2316 21 -1.5867000219675873 2316 22 -1.5453754439327483 2316 23 -1.4478859052620408 2316 24 -1.5635634986176399 2316 25 -1.4283790856907075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10092.0 3 0.0 4 249.0 5 1701.0 6 2097.0 7 1690.0 8 1400.0 9 1247.0 10 1092.0 11 1101.0 12 1059.0 13 1074.0 14 981.0 15 969.0 16 1025.0 17 1068.0 18 1213.0 19 1427.0 20 1790.0 21 2295.0 22 2920.0 23 3721.0 24 4475.0 25 5388.0 26 6352.0 27 7252.0 28 8742.0 29 10352.0 30 13255.0 31 17545.0 32 22836.0 33 30542.0 34 41538.0 35 57235.0 36 86199.0 37 160347.0 38 685394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.167113397062785 19.657419375335465 13.090984183570242 25.084483044031508 2 14.878935494052401 21.203948478072455 40.50663562748304 23.41048040039211 3 18.3493132784535 25.53373406354293 30.609269676290257 25.50768298171331 4 12.358081337335767 17.800610384893183 38.482053765640586 31.35925451213046 5 12.652282536153137 38.370312704905366 36.17736065599787 12.80004410294362 6 30.983576983723975 37.924857016863996 18.214815458214183 12.87675054119785 7 26.977935115660884 32.24123805873113 23.01617724062424 17.76464958498375 8 24.800436913513337 36.920778527771816 21.08383278037163 17.19495177834322 9 26.416950460388854 14.776619962487453 20.684261599076716 38.12216797804698 10 14.732545893836809 29.096538033361085 35.25004113136732 20.92087494143478 11 33.345137473691246 22.443457054087464 25.43104600273945 18.78035946948184 12 22.939833258999787 25.241500501960235 32.24630963179004 19.572356607249944 13 28.714974990040282 22.301500931661717 26.189006642317903 22.7945174359801 14 20.51983081490265 22.637984810084298 28.33340988137837 28.508774493634682 15 22.646197169924772 31.329838052352383 23.915588797284837 22.10837598043801 16 21.41436866415634 28.35626951537973 28.603961909152176 21.62539991131176 17 21.209451475383826 28.998526077570908 28.364363645473638 21.427658801571628 18 21.02908352128636 27.48609453793617 30.631346806941835 20.853475133835637 19 22.820429964772178 27.23530497239138 28.628386158364037 21.315878904472406 20 23.12984072167875 27.452214833691258 28.971604439352998 20.44634000527699 21 22.305301100398527 26.798407223437092 28.397362516981072 22.498929159183305 22 22.572222528385513 28.358291208047977 28.09543610733974 20.974050156226774 23 21.629759267624497 27.951457745543934 28.8178304444502 21.60095254238137 24 22.57650260220317 27.16644999035602 29.181581428126506 21.075465979314302 25 22.03860521582884 27.586330700982415 28.787086840861615 21.587977242327135 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 388.0 1 388.0 2 491.0 3 594.0 4 594.0 5 594.0 6 1379.5 7 2165.0 8 2165.0 9 2165.0 10 2832.0 11 3499.0 12 3499.0 13 3499.0 14 4988.5 15 6478.0 16 6478.0 17 6478.0 18 11422.5 19 16367.0 20 16367.0 21 16367.0 22 25800.0 23 35233.0 24 35233.0 25 35233.0 26 49537.5 27 63842.0 28 63842.0 29 63842.0 30 80864.5 31 97887.0 32 97887.0 33 97887.0 34 109040.5 35 120194.0 36 120194.0 37 120194.0 38 127749.5 39 135305.0 40 135305.0 41 135305.0 42 140966.0 43 146627.0 44 146627.0 45 146627.0 46 151013.0 47 155399.0 48 155399.0 49 155399.0 50 148426.0 51 141453.0 52 141453.0 53 141453.0 54 124673.0 55 107893.0 56 107893.0 57 107893.0 58 91406.0 59 74919.0 60 74919.0 61 74919.0 62 60692.5 63 46466.0 64 46466.0 65 46466.0 66 35741.0 67 25016.0 68 25016.0 69 25016.0 70 18062.5 71 11109.0 72 11109.0 73 11109.0 74 7776.0 75 4443.0 76 4443.0 77 4443.0 78 3030.0 79 1617.0 80 1617.0 81 1617.0 82 1090.5 83 564.0 84 564.0 85 564.0 86 365.0 87 166.0 88 166.0 89 166.0 90 99.0 91 32.0 92 32.0 93 32.0 94 18.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13067114872881605 2 0.0030893498421509223 3 0.0013359350668760745 4 0.00484276461742577 5 0.03874211693940616 6 0.026635205395841736 7 0.020039026003141117 8 0.0143613019689178 9 0.016448700510911667 10 0.023378863670331304 11 0.028639107996155846 12 0.029808051179672414 13 0.028388620171116586 14 0.013192358785401237 15 0.019955530061461363 16 0.017534147752748478 17 0.01486227761899633 18 0.009351545468132522 19 0.0025048782503926397 20 9.184553584773012E-4 21 5.00975650078528E-4 22 0.0037573173755889593 23 0.002170894483673621 24 0.002003902600314112 25 8.349594167975465E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1197663.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.98944445134065 #Duplication Level Percentage of deduplicated Percentage of total 1 86.81852413545043 65.97291417138189 2 9.69872553731463 14.740015309331381 3 1.9484684545120496 4.441891061679989 4 0.62592950707242 1.9025614203253878 5 0.2619487546355659 0.9952670169738597 6 0.15056597349390866 0.686485480744644 7 0.09905554956445264 0.5269023326857496 8 0.06288253953447165 0.3822727395931172 9 0.054725702326535466 0.3742718145302584 >10 0.24397977494380962 3.2718631131091254 >50 0.016296281838618885 0.8734445293063621 >100 0.01560167699707701 2.500195499237383 >500 0.0018677969790749896 0.9764145510254884 >1k 0.001318444926405875 1.9308712545161582 >5k 1.0987041053382291E-4 0.4246297055592676 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5086 0.42466035938323216 No Hit GGTATCAACGCAGAGTACTTTTTTT 3503 0.29248628370418056 No Hit TATCAACGCAGAGTACTTTTTTTTT 3322 0.277373518260145 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1983 0.1655724523509535 No Hit CTGTAGGACGTGGAATATGGCAAGA 1977 0.16507147670087494 No Hit GTCCTACAGTGGACATTTCTAAATT 1896 0.1583083054248148 No Hit CTTTAGGACGTGAAATATGGCGAGG 1830 0.15279757327395102 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1755 0.14653537764796942 No Hit GTACATGGGAAGCAGTGGTATCAAC 1728 0.14428098722261604 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1470 0.12273903426923934 No Hit CCCATGTACTCTGCGTTGATACCAC 1302 0.10871171606704057 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1198 0.10002813813234608 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 8.349594167975465E-5 0.0 11 0.0 0.0 0.0 8.349594167975465E-5 0.0 12 0.0 0.0 0.0 8.349594167975465E-5 0.0 13 0.0 0.0 0.0 8.349594167975465E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 425 0.0 13.414596 4 AGGACCT 710 0.0 11.37564 5 GGACCTG 690 0.0 11.151733 6 CCGTGTC 60 0.005874226 11.083358 9 GACGTGA 710 0.0 10.8376 7 CTAGCAC 80 3.7634172E-4 10.68931 3 GGCGAGG 615 0.0 10.653665 19 AATGTCC 385 0.0 10.609969 8 TGGGTTG 155 3.6434358E-9 10.421555 5 TTAGGAC 870 0.0 10.3753195 3 CCACCTT 325 0.0 10.229083 13 ACGAAAT 85 6.590452E-4 10.0588455 12 GAAATGT 425 0.0 10.058426 6 ACTTATC 95 1.643762E-4 9.999604 8 TGGCGAG 1290 0.0 9.938146 18 ATGTCCA 365 0.0 9.890432 9 GTCCTAC 1040 0.0 9.787962 1 TTTAGGA 935 0.0 9.755663 2 TTTAGAA 565 0.0 9.753863 2 AGGACGT 1210 0.0 9.737593 5 >>END_MODULE