FastQCFastQC Report
Thu 26 May 2016
SRR1512503_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512503_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479425
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46610.3150548354935194No Hit
GTACATGGGAAGCAGTGGTATCAAC34280.23171164472683645No Hit
GGTATCAACGCAGAGTACTTTTTTT29900.2021055477634892No Hit
TATCAACGCAGAGTACTTTTTTTTT27110.1832468695608091No Hit
CCCATGTACTCTGCGTTGATACCAC25680.173580952059077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG500.001526379313.2686545
GTCCTAC7050.011.6815481
GTGTACG600.00424833311.5759911
ACGAAAT1105.1049756E-711.20347612
CGAAATC855.415891E-511.15733113
TAGACAC1303.3796823E-810.9357035
GTCTATC1001.4826084E-510.9145071
TGTAGGA9100.010.7277692
CGTGCAC803.8498928E-410.66339810
AGGACGT9800.010.4447135
GTAGGAC8850.010.3878883
GTCTAGT1156.112412E-610.3536821
TAGGACC2850.010.3089784
GACGTGG5350.010.2747917
TAGGACT1951.6370905E-1110.2066564
GGACGTG9600.010.1686856
ACGTGGA5600.010.1267128
TGTACCG750.00269975410.109455
ACCGTAT750.002761996410.0817048
AGGACCG856.73705E-410.0351165
Produced by FastQC (version 0.11.5)