FastQCFastQC Report
Thu 26 May 2016
SRR1512503_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512503_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479425
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55350.37413184176284703No Hit
GTACATGGGAAGCAGTGGTATCAAC39710.268415093701945No Hit
GGTATCAACGCAGAGTACTTTTTTT39180.26483262078172265No Hit
TATCAACGCAGAGTACTTTTTTTTT38200.2582084255707454No Hit
CCCATGTACTCTGCGTTGATACCAC29130.19690082295486422No Hit
GAGTACATGGGAAGCAGTGGTATCA17800.12031701505652534No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17410.11768085573787113No Hit
CTGTAGGACGTGGAATATGGCAAGA16600.1122057556145124No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16470.11132703584162766No Hit
GTCCTACAGTGGACATTTCTAAATT16060.10855568886560657No Hit
GTCCTAAAGTGTGTATTTCTCATTT15560.1051759974314345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC350.002170700816.2850768
CAAGTCG803.7706282E-410.6874436
AGGACGT9000.010.5576415
TCTATAC1355.673246E-810.5569273
GGACGTG9100.010.2307156
TATACTG2350.010.1083795
TAGGACC2450.010.0836244
GCCTTAC856.5032294E-410.0737561
TAGGACG9350.010.0608114
AAGTCGT951.6443494E-49.9996097
GGCGAGG4300.09.93878319
GTCCTAA5100.09.8872061
GTAGGAC7800.09.8666663
GGTCTAC1356.3095285E-79.8664781
TCAGTAC1554.051799E-89.8077263
GACGTGG4950.09.7874967
TGGCGAG8450.09.77803718
TAGGTTG800.0045078799.5018775
TTAGACT1201.6956903E-59.5018764
TTATACT2600.09.5018764
Produced by FastQC (version 0.11.5)