FastQCFastQC Report
Thu 26 May 2016
SRR1512502_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512502_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424901
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT98190.28669441832041276No Hit
CTGTAGGACGTGGAATATGGCAAGA87620.2558322123763578No Hit
GTCCTAAAGTGTGTATTTCTCATTT85550.2497882420542959No Hit
CTTTAGGACGTGAAATATGGCGAGG71400.20847317922474254No Hit
GTATCAACGCAGAGTACTTTTTTTT63250.1846768709518903No Hit
GTCCTACAGTGTGCATTTCTCATTT59350.1732896804900346No Hit
GTACATGGGAAGCAGTGGTATCAAC57290.1672749080922339No Hit
CCCATGTACTCTGCGTTGATACCAC41430.12096700021402079No Hit
CTGTAGGACCTGGAATATGGCGAGA39210.11448506102804139No Hit
GGTATCAACGCAGAGTACTTTTTTT37950.11080612257113417No Hit
TATCAACGCAGAGTACTTTTTTTTT37920.11071852879835067No Hit
CTGAAGGACCTGGAATATGGCGAGA37860.11054334125278366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC19450.013.4961554
GTACGGG450.00871822613.2106231
TCCAACG5400.012.80280318
CCAACGT2950.011.88060219
GGCGAGG23050.011.62985419
GTGTAGG6850.011.5713481
CCAACGA3250.011.36684619
CCACCTT13250.011.30912213
GTCCTAC51350.011.2875571
CCGTCCA855.4668602E-511.1480549
TACCGGG600.005987254411.0559647
ATCCTAC4500.011.0088521
AGGACCT36750.010.9850535
GGACCTG35200.010.9303286
TCCTACA58650.010.8746682
TAGGCCG700.001523284110.8303335
TGTAGGA57100.010.7715342
ACCTTTT13900.010.71771415
CACCTTT13700.010.66638914
TACACCG803.8603417E-410.6611095
Produced by FastQC (version 0.11.5)