FastQCFastQC Report
Thu 26 May 2016
SRR1512502_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512502_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424901
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT107250.3131477377010314No Hit
CTGTAGGACGTGGAATATGGCAAGA106710.31157104979092826No Hit
GTCCTAAAGTGTGTATTTCTCATTT91350.2667230381257735No Hit
CTTTAGGACGTGAAATATGGCGAGG88930.2596571404545708No Hit
GTATCAACGCAGAGTACTTTTTTTT81910.23916019762323057No Hit
GTACATGGGAAGCAGTGGTATCAAC68920.2012320940079728No Hit
GTCCTACAGTGTGCATTTCTCATTT64950.1896405180762889No Hit
TATCAACGCAGAGTACTTTTTTTTT56250.1642383239690724No Hit
GGTATCAACGCAGAGTACTTTTTTT53860.15726002007065315No Hit
CTGTAGGACCTGGAATATGGCGAGA49490.14450052716852255No Hit
CCCATGTACTCTGCGTTGATACCAC49070.14327421434955345No Hit
CTGAAGGACCTGGAATATGGCGAGA47170.13772660873993145No Hit
TTTCTAAATTTTCCACCTTTTTCAG36830.10753595505388332No Hit
ATTTAGAAATGTCCACTGTAGGACG35720.10429498546089362No Hit
TTGTAGAACAGTGTATATCAATGAG35460.10353583943010322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC17600.013.6586034
CCAACGA3100.013.47890819
TCCAACG4950.012.0873418
GGCGAGG22550.012.04434719
AGGACCT34000.011.3186215
GGACCTG33600.011.13935856
CCGTGTA600.005871215411.085082
TGTAGGA57100.010.9824672
ATCCCGT3750.010.892982510
ACCTTTT13550.010.86553915
GGACCGT700.001492780310.8566336
CTGTAGG57450.010.7272791
TCCCGTT3900.010.71760311
AAATGTC14650.010.6991577
GGACGTG54700.010.6283866
GTAGGAC56750.010.6148883
AGGACGT56000.010.5880015
TGGCGAG54400.010.57959718
TAGAAAT14550.010.5138384
GACGTGG27700.010.4597997
Produced by FastQC (version 0.11.5)