FastQCFastQC Report
Thu 26 May 2016
SRR1512501_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512501_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649086
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55070.3339425596967047No Hit
GTACATGGGAAGCAGTGGTATCAAC38880.2357669642456488No Hit
GTCCTACAGTGGACATTTCTAAATT38250.2319466662138906No Hit
CTGTAGGACGTGGAATATGGCAAGA36630.2221230427036552No Hit
GTCCTAAAGTGTGTATTTCTCATTT36480.221213447934189No Hit
GGTATCAACGCAGAGTACTTTTTTT35740.21672611373815556No Hit
TATCAACGCAGAGTACTTTTTTTTT31810.19289473077814012No Hit
CTTTAGGACGTGAAATATGGCGAGG30980.18786163972042697No Hit
CCCATGTACTCTGCGTTGATACCAC27370.1659707256019395No Hit
GAGTACATGGGAAGCAGTGGTATCA17080.1035725244165556No Hit
GTCCTACAGTGTGCATTTCTCATTT16820.10199589348281411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC655.5659613E-513.12508910
CGGTTTC1207.6033757E-1012.64475513
GGCGTGC1207.9671736E-1012.6078528
GCCGGTT1251.4606485E-912.13341511
GGGACCG550.00312426412.0619035
TAGGACC6000.012.0044644
CTCGAAC1201.0502845E-811.83677418
GCCTAAT853.4445555E-511.6635351
CCCTTAT1052.077115E-611.3302921
GTTCTAA1501.0531949E-911.2358721
GCGTGCG855.4633347E-511.1479599
CCGGTTT1201.3126737E-711.06011412
AAAGGCG1053.5671655E-610.8310965
TCCAACG1408.780262E-910.82219318
GCGCCAC1159.014493E-710.72055313
TGCCGGT1252.3082248E-710.61673810
AAGGCGT1252.3150278E-710.6144736
GTCTTAA2150.010.605681
TGTAGGA23100.010.4624912
GTCCTAA11300.010.44041251
Produced by FastQC (version 0.11.5)