FastQCFastQC Report
Thu 26 May 2016
SRR1512501_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512501_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649086
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69530.42162749547325007No Hit
TATCAACGCAGAGTACTTTTTTTTT48920.296649174148589No Hit
GGTATCAACGCAGAGTACTTTTTTT48130.2918586416960668No Hit
GTACATGGGAAGCAGTGGTATCAAC45970.27876047701575296No Hit
CTGTAGGACGTGGAATATGGCAAGA44930.27245395328078703No Hit
GTCCTACAGTGGACATTTCTAAATT41920.25420141824016457No Hit
GTCCTAAAGTGTGTATTTCTCATTT39220.23782871238977227No Hit
CTTTAGGACGTGAAATATGGCGAGG38240.23188602656259286No Hit
CCCATGTACTCTGCGTTGATACCAC33200.20132364230852728No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22840.1385009635640591No Hit
CTGAAGGACCTGGAATATGGCGAGA21170.128374141797335No Hit
GAGTACATGGGAAGCAGTGGTATCA20420.12382616795000381No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18670.11321422897289772No Hit
GTCCTACAGTGTGCATTTCTCATTT18440.11181951699304948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG401.2808347E-519.0076475
AGCCGCG350.00217235816.2833757
ACCGTGC551.9626568E-413.8161968
TAGGACC5500.012.9597594
GTCTAAT1103.741843E-812.1075321
TCCAACG1353.7289283E-1011.95943118
GCCGGTT1201.00353645E-811.87329411
CGGTTTC1451.2223609E-911.13531913
GGACGTG23200.011.0953896
AGGACGT24000.011.0877945
ACAGTCC951.3623901E-510.998428
GGCGAGG10400.010.95763719
TGTAGGA23450.010.8203282
GACGTGG12550.010.747467
CTGTAGG23000.010.6298111
TGTCCAC6250.010.48649310
GTCCTAT1855.820766E-1110.28438951
AATGTCC6300.010.2524968
AATCCCG1303.8593134E-710.22712819
GACTGTC1409.7059456E-810.177117
Produced by FastQC (version 0.11.5)