FastQCFastQC Report
Thu 26 May 2016
SRR1512500_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512500_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128000
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74290.2375No Hit
CTGTAGGACGTGGAATATGGCAAGA68560.21918158567774937No Hit
GTCCTAAAGTGTGTATTTCTCATTT68250.21819053708439898No Hit
GTATCAACGCAGAGTACTTTTTTTT57640.18427109974424552No Hit
CTTTAGGACGTGAAATATGGCGAGG56870.18180946291560102No Hit
GTCCTACAGTGTGCATTTCTCATTT42340.13535805626598466No Hit
GTACATGGGAAGCAGTGGTATCAAC41740.13343989769820971No Hit
GGTATCAACGCAGAGTACTTTTTTT35620.11387468030690537No Hit
TATCAACGCAGAGTACTTTTTTTTT34650.11077365728900256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA450.00873877413.2058621
TAGGACC13650.013.0535074
TCCAACG3200.011.83949118
TAAGACG907.66525E-611.583834
TAGGACT6350.011.3433644
CGTATCA700.001053256711.319311
GGCGAGG19250.011.16927819
GGACCTG25200.010.9444636
GTACCGT1053.5683988E-610.8316346
TGTAGGA45000.010.8254422
AAGGCGT1502.220986E-910.741376
GTCCTAC40950.010.6904591
AGGACCT27350.010.6732195
CGTAGGA803.8564327E-410.6622172
ACTGTTC8900.010.6241618
GTAGGAC44500.010.4786923
TGGCGAG41750.010.45845418
ACCGTGC1451.5548721E-810.4336598
TTAGGAC26900.010.3585043
AGGACGT43200.010.3113635
Produced by FastQC (version 0.11.5)