FastQCFastQC Report
Thu 26 May 2016
SRR1512500_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512500_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3128000
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA83260.2661764705882353No Hit
GTCCTACAGTGGACATTTCTAAATT82610.26409846547314575No Hit
GTCCTAAAGTGTGTATTTCTCATTT76210.2436381074168798No Hit
GTATCAACGCAGAGTACTTTTTTTT76070.243190537084399No Hit
CTTTAGGACGTGAAATATGGCGAGG69270.22145140664961635No Hit
TATCAACGCAGAGTACTTTTTTTTT52660.16835038363171356No Hit
GTACATGGGAAGCAGTGGTATCAAC50100.16016624040920716No Hit
GGTATCAACGCAGAGTACTTTTTTT49850.15936700767263426No Hit
GTCCTACAGTGTGCATTTCTCATTT49560.1584398976982097No Hit
CTGAAGGACCTGGAATATGGCGAGA38680.12365728900255754No Hit
CTGTAGGACCTGGAATATGGCGAGA36750.11748721227621484No Hit
CCCATGTACTCTGCGTTGATACCAC36210.11576086956521739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15050.013.5750064
TCCAACG3150.011.45637318
TCCGCTC752.0758732E-411.399548
AGGACCT29100.011.298695
GGCGAGG19700.011.23109619
GGCGTGC1408.367351E-910.8567058
CGTGCGC1053.47095E-610.85670410
TGTAGGA48500.010.8540592
GGACCTG29050.010.8240036
CCAACGA2050.010.65384719
GTAGGAC46500.010.646553
GGACGTG44750.010.571646
GACGTGG22650.010.5271687
CTGTAGG47300.010.4782151
AGGACGT45650.010.4081665
TGTAGAA11650.010.3586222
CCACCTT11150.010.30784713
GTGTAGG5650.010.2705121
AAATGTC10250.010.1947117
AATCCGC750.002649740310.13292410
Produced by FastQC (version 0.11.5)