FastQCFastQC Report
Thu 26 May 2016
SRR1512499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899195
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC80940.27918094505543783No Hit
GTCCTACAGTGGACATTTCTAAATT62980.21723271459836266No Hit
CCCATGTACTCTGCGTTGATACCAC59800.20626415263547293No Hit
GTATCAACGCAGAGTACTTTTTTTT58330.20119377965262775No Hit
GTCCTAAAGTGTGTATTTCTCATTT55750.19229475768273607No Hit
CTGTAGGACGTGGAATATGGCAAGA55490.1913979570191036No Hit
CTTTAGGACGTGAAATATGGCGAGG46830.1615275964534983No Hit
GTCCTACAGTGTGCATTTCTCATTT36100.12451732291204973No Hit
GGTATCAACGCAGAGTACTTTTTTT35340.1218959055875855No Hit
GAGTACATGGGAAGCAGTGGTATCA34910.12041273525927024No Hit
TATCAACGCAGAGTACTTTTTTTTT34890.12034375059283699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAG450.008714803513.2113071
TAGGACC11550.013.0471094
GTTTGCG550.003129518612.0597059
ATGACCG658.1874785E-411.6647595
GTCTTAG3100.011.1869941
GACGTGG18350.011.1045647
GTGCGAG600.005978738411.05798611
GAACCGA600.00598182411.0572196
GGCGAGG16000.011.01009719
TGTAGGA39200.010.8801052
GGACGTG35900.010.8504186
AGGACGT36100.010.84281355
GTTTAGG3200.010.83739951
GTCCTAC32400.010.8259321
GTAGGAC38300.010.6654123
ACGTGGA19200.010.6359598
TAGAAAT10900.010.6079744
AATCCCG2150.010.57233619
CGTATCA1052.5840867E-510.3803131
GTCCTAT3750.010.3048191
Produced by FastQC (version 0.11.5)