FastQCFastQC Report
Thu 26 May 2016
SRR1512499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2899195
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC96090.33143682987863876No Hit
GTATCAACGCAGAGTACTTTTTTTT76990.26555647343486727No Hit
CCCATGTACTCTGCGTTGATACCAC70100.24179125584860625No Hit
CTGTAGGACGTGGAATATGGCAAGA68650.23678986753219428No Hit
GTCCTACAGTGGACATTTCTAAATT68360.23578958986891188No Hit
GTCCTAAAGTGTGTATTTCTCATTT60250.2078163076302215No Hit
CTTTAGGACGTGAAATATGGCGAGG56490.194847190340767No Hit
TATCAACGCAGAGTACTTTTTTTTT50760.17508308340763556No Hit
GGTATCAACGCAGAGTACTTTTTTT49390.17035763375695667No Hit
GAGTACATGGGAAGCAGTGGTATCA42480.1465234315042624No Hit
GTCCTACAGTGTGCATTTCTCATTT38850.13400271454662413No Hit
CTGAAGGACCTGGAATATGGCGAGA30990.10689174063834961No Hit
CTGTAGGACCTGGAATATGGCGAGA30500.10520161631073453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11300.014.2973074
GGCGAGG15850.011.74244119
AGGACCT23700.011.3085535
GGACCTG23150.010.9145176
CGACAAA700.001495006410.85458914
TGGCGAA1502.1482265E-910.763020518
TCCAACG1803.6379788E-1110.55198118
GTAGGAC36250.010.5386933
TGGCGAG35300.010.51909918
CCAACGA1002.4120784E-510.4453819
CCTATAC2750.010.3671013
TGTAGGA37500.010.2379112
ATAGGAC3450.010.19181253
TAGGACA10700.010.1252294
GGACGTG36800.010.1184076
CCTACAC3200.010.0971253
AGGACGT37100.010.0931925
GGCGAAA951.6522923E-49.9955819
TTACACG1054.0909275E-59.9560524
TTAGGAC23300.09.9518243
Produced by FastQC (version 0.11.5)